Member since: 2018
Organization: University of Greifswald
Great results with OptiPrep for purification of outer membrane vesicles
Application Area: Proteomics
"I have been using OptiPrep for the purification of outer membrane vesicles via density gradient centrifugation for quite some time now. I am very satisfied with the product, expecially for the value when compared to other vendors."
OptiPrep™ is a ready-made, sterile and endotoxin-tested solution of iodixanol. Suitable for the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendritic cells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes, endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients. Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins. Please note we are the official distributor for Germany, please contact your local distributor for orders if your place of business is located outside of Germany.