200 cm µPAC™ column

In this application note, PharmaFluidics describes optimal LC methods for a variety of LC-MS based proteome analyses. This application note highlights PharmaFluidics' µPAC™ capLC column as a column that promises to maximize throughput of routine proteome analysis.

In this application note, PharmaFluidics uses micropillar array columns (µPAC™) in combination with mass spectrometry for the characterization of host cell proteins and their monitoring during downstream processing.

Learn more about µPAC™ micro-Chip based HPLC columns from PharmaFluidics

Liquid chromatography coupled with mass spectrometry is a powerful analytical tool for detection and identification of chemicals in complex mixtures. With the low flow capability of Nano LC-MS, higher resolution, ion suppression tolerance and ionisation efficiency is obtained which gives greater chemical sensitivity. The low flow capability of nano-LC couples perfectly with the microfluidics of the Microsaic 4500 MiD® mass detector. The reduced solvent, nitrogen and power consumption of the Microsaic 4500 MiD® provides a lower cost and greener solution for mass detection.

Discover 6 key reasons why you should use µPAC™ columns for your your separations.

Peptide standards are critical in mass spectrometry based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis. They can be used to assess peptide elution, troubleshoot chromatography, predict retention time, and to demonstrate that the LC-MS platforms are working properly.

The Pharmafluidics μPAC™ column's micromachined micro-pillar array technology provides distinct advantages over competitors. In this application note, Pharmafluidics discusses how sharp separation peaks, low backpressure, and resilience that allow the μPAC column to boost laboratory output.

In this application note, Pharmafluidics demonstrates the linkage of the Microsaic 4500 MiD® to the μPAC™ column, producing a compact and high-res platform for separations.

In part 2 of this technical note, you will find further analysis of performance and efficiency in the μPACTM column, including peak gain, width, and capacity.

To see part 1, click here >>

Ceramides, a type of sphingolipid, form a key role in skin permeability in the human body. Due to the structural diversity present, profiling ceramides requires high-fidelity chromatographic separation. In this note, the μPAC™ column is used to successfully perform this separation

Ir. Paul Jacobs, COO of PharmaFluidics, discusses its new micropillar array ‘trapping’ column launched at Pittcon 2018. This novel technology complements the already existing analytical column in chromatographic systems to enable higher flow rates, sample enrichment, and sample de-salting. Coupled with state-of-the-art mass spectrometry platforms, the trapping column will bring highly efficient and reproducible biomolecular separation to the user.

In this video guide, learn how to set-up the µPAC™ column for performing HPLC. The µPAC is compatible with industry standard connections, making setup fast and simple.

In this video, discover how the µPAC™’s micromachined separation bed technology produces a miniaturized, high-performance separation tool compatible with all Nano-LC-MS equipment. The µPAC's design makes it ideal for small volumes of complex samples, such as in biomarker identification and biopharmaceutical development.

The technology extends capabilities in column performance for biopharma and proteomics researchers

Infectious disease expert shares how robust LC columns are facilitating the analysis of complex pathogen samples

We talk with the team at PharmaFluidics about how their recent changes in sourcing and production allows them to face demand from a growing community of users

Take a look back through 2019 at the best content from our analytical community

Look back at some of the best life sciences content SelectScience has covered in 2019

The latest research and new method downloads: multiplex assays, ELISA, proteomics, genomics, imaging and more

From optimized sample prep, imaging, and analysis to mapping the entire human proteome, discover the latest technologies, top tips, and best practices for protein biology research

SelectScience speaks to the President of the German Society for Mass Spectrometry, who is using chemical cross-linking and mass spectrometry to provide deeper proteomics insights

Watch this on-demand expert webinar on how to use µPAC™ to improve the sensitivity of single-cell proteomic samples