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Ceramide Profiling Using an μPAC™ Micro-Array Column Combined with High-Res Mass Spectrometry

PharmaFluidics
4 Jul 2018

Ceramides, a type of sphingolipid, form a key role in skin permeability in the human body. Due to the structural diversity present, profiling ceramides requires high-fidelity chromatographic separation. In this note, the μPAC™ column is used to successfully perform this separation

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50 cm µPAC™ column

PharmaFluidics

With an internal volume of 3µL, this 50 cm long RP-C18 µPAC™ column is perfectly suited to perform high throughput analyses with shorter gradi­ent solvent times (30, 60 and 90-minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min. µPAC™ column, 50 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.

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200 cm µPAC™ column

PharmaFluidics

As an alternative to conventional columns, PharmaFluidics offers micromachined nano-LC columns or micro Pillar Array Columns (µPAC™). These µPAC™ columns display exceptional separation properties with unprecedented peak capacity and narrow peak shapes at low operating back-pressure. µPAC™ column, 200 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.

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Ceramide Profiling Using an μPAC™ Micro-Array Column Combined with High-Res Mass Spectrometry