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XenoWorks Digital Microinjector

Designed primarily for transgenic mammal workstations, the XenoWorks Digital Microinjector is capable of holding oocytes and early embryos immobile while simultaneously providing a separate pressure channel for pronuclear microinjection of DNA or the gentle control of embryonic stem cells as they are transferred into blastocysts. Features of the XenoWorks Digital Microinjector:• Built-in compressor with remote user interface •…

Sutter Instrument Co.

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Description

Designed primarily for transgenic mammal workstations, the XenoWorks Digital Microinjector is capable of holding oocytes and early embryos immobile while simultaneously providing a separate pressure channel for pronuclear microinjection of DNA or the gentle control of embryonic stem cells as they are transferred into blastocysts.

Features of the XenoWorks Digital Microinjector:

• Built-in compressor with remote user interface
• Two independent pressure channels
• Negative and positive pressure
• Two injection modes: pulse and continuous
• Four functions: Hold, Transfer, Inject, Clear
• Transfer channel provides compensation pressure for high pressure injection
• Four range settings for transfer/compensation pr
• Rotary controls for continuous pressure adjustment

Common Applications of the XenoWorks Digital Microinjector:

• Zygote pronuclear DNA microinjection
• Embryonic stem cell transfer into blastocyst
• Intracytoplasmic Sperm Injection (ICSI)
• Piezo-assisted ICSI
• Microinjection of cultured, adherent cells
• Somatic cell nuclear transfer

Application NoteLife Sciences

Introduction to Single-Cell Microinjection

Single-cell microinjection is a powerful and versatile technique for introducing exogenous material into cells and for extracting and transferring cellular components between cells. This application note describes the key points you should take into consideration when carrying out microinjection, including choosing a suitable workplace, microscope and micromanipulator. It also discusses potential applications of microinjection and suggested system configurations for each application.


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