Introduction to Single-Cell Microinjection

Designed primarily for transgenic mammal workstations, the XenoWorks Digital Microinjector is capable of holding oocytes and early embryos immobile while simultaneously providing a separate pressure channel for pronuclear microinjection of DNA or the gentle control of embryonic stem cells as they are transferred into blastocysts. Features of the XenoWorks Digital Microinjector:• Built-in compressor with remote user interface • Two independent pressure channels • Negative and positive pressure • Two injection modes: pulse and continuous • Four functions: Hold, Transfer, Inject, Clear • Transfer channel provides compensation pressure for high pressure injection • Four range settings for transfer/compensation pr • Rotary controls for continuous pressure adjustment Common Applications of the XenoWorks Digital Microinjector: • Zygote pronuclear DNA microinjection • Embryonic stem cell transfer into blastocyst • Intracytoplasmic Sperm Injection (ICSI) • Piezo-assisted ICSI • Microinjection of cultured, adherent cells • Somatic cell nuclear transfer

XenoWorks, a modular system designed to meet a wide variety of microinjection application needs. Our experience with precision motor control has culminated in this state of the art system. Features:XenoWorks Micromanipulator: Exceptionally smooth and responsive micropipette movement Six movement sensitivities Height- and tension-adjustable inverted joystick for optimal ergonomics Up to 25mm of movement in three axes User defined “Work” and Z-axis limit XenoWorks Digital Microinjector: Independent “hold” and inject/transfer channels Femtoliter resolution injection Up to 5,600 hPa of injection pressure Remote keypad with rotary controls and high-visibility LED displays XenoWorks Analog Microinjector: Coarse and fine control Use with a variety of hydraulic fluids, including oil, air and water Interchangeable syringes allow sensitivity to be modified