What is “Filamented” Glass & Who Needs It?

Designed primarily for transgenic mammal workstations, the XenoWorks Digital Microinjector is capable of holding oocytes and early embryos immobile while simultaneously providing a separate pressure channel for pronuclear microinjection of DNA or the gentle control of embryonic stem cells as they are transferred into blastocysts. Features of the XenoWorks Digital Microinjector:• Built-in compressor with remote user interface • Two independent pressure channels • Negative and positive pressure • Two injection modes: pulse and continuous • Four functions: Hold, Transfer, Inject, Clear • Transfer channel provides compensation pressure for high pressure injection • Four range settings for transfer/compensation pr • Rotary controls for continuous pressure adjustment Common Applications of the XenoWorks Digital Microinjector: • Zygote pronuclear DNA microinjection • Embryonic stem cell transfer into blastocyst • Intracytoplasmic Sperm Injection (ICSI) • Piezo-assisted ICSI • Microinjection of cultured, adherent cells • Somatic cell nuclear transfer

The Model P-97 micropipette puller is the latest generation of the Flaming/Brown type puller for fabrication of micropipettes, patch pipettes and microinjection needles. While retaining many of the features on the Model P-87, the P-97 offers improvements in mechanical, electronic and software design. The result is better control of the pulling process and a higher degree of reproducibility. Features: Environmental chamber Programmable air pressure Memory storage for up to 100 programs Write protection and date stamp for each program Two symmetrical pipettes with each pull Two cooling modes: time and delay Preprogrammed sample programs for intracellular and patch pipettes. Special programming on request. Ramp test—self test for establishing program heat settings when a new filament or glass is introduced. Vacuum fluorescent display Constant current power supply for filament and pull solenoid Looping pull cycle for fabrication of patch type micropipettes Self-contained air supply with filtration system and humidity control Consistent and reliable electrodes with tip diameters less than 0.1µm Control over the time and pressure at which the air is delivered Optimized velocity sensing circuit for maximized sensitivity and reproducibility Quality control, SEM photograph of a tip pulled with each puller; criterion is tip measurement less than 0.1µm and typically is ~0.06µm