VersaMAP™ Magnetic Custom Multiplex Kits
The VersaMAP™ Magnetic Custom Multiplex Kits are our most efficient Luminex-based multiplex assays. These homogeneous, no wash assays utilize analyte-specific antibodies conjugated to superparamagnetic microparticles to simultaneously detect up to 25 user-defined phosphorylated receptor tyrosine kinases (RTKs) in a single sample. Available Kits VersaMAP Magnetic Human RTK Kit A VersaMAP Magnetic Human RTK Kit B Features • Comp…
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The VersaMAP™ Magnetic Custom Multiplex Kits are our most efficient Luminex-based multiplex assays. These homogeneous, no wash assays utilize analyte-specific antibodies conjugated to superparamagnetic microparticles to simultaneously detect up to 25 user-defined phosphorylated receptor tyrosine kinases (RTKs) in a single sample.
AvailableKits
VersaMAP Magnetic Human RTK Kit A
VersaMAP Magnetic Human RTK Kit B
Features
• Complete ready-to-use kits
• Microplate-based assay
• User defines up to 25 analytes of interest
• Simultaneous detection of selected analytes
• Small sample size (1-5 μg of cell lysate)
• Simple procedure: no washing required
• Superparamagnetic beads are premixed
• Compatible with multiple analyzing systems*
Kit Contents
• Premixed antibody-coated superparamagnetic bead cocktail
• Biotinylated anti-phospho-tyrosine detection antibody
• Positive Recombinant Peptide Control
• Streptavidin-PE
• Diluents
• Lysis Buffer
• 1 Round-bottomed 96-well Microplate
• Foil Plate Sealers
• Mixing Bottle
*Compatible with Luminex MAGPIX®, Luminex 200™, and Bio-Rad® Bio-Plex® analyzers.
A Homogeneous Multiplex Immunoassay for RTK Phosphorylation State that is Adaptable to HTS
This application note looks at the VersaMAP™ Magnetic Human RTK Multiplex Kits which are Luminex ® bead-based multiplex immunoassays for the analysis of RTK phosphorylation. These assays can be readily adapted for high-throughput screening with minimal manual intervention from cell treatment to data collection. To demonstrate this, several different cell lines were grown in 96-well plates, treated with various stimulants or inhibitors, and lysed in the same plate.
