Protein Reagents
Stable Isotope-labeled Reagents
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To support the intricacies of protein biology, CIL offers a wide range of isotope-labeled reagents that serve to stabilize, simplify, or expedite research in this field. This includes reagents for use with 1H-NMR, reverse micelles, dynamic nuclear polarization (DNP), 17O labeling, and other applications.
Uniform isotope labeling of eukaryotic proteins in methylotrophic yeast for high-resolution NMR studies
In this application note, Cambridge Isotope Laboratories demonstrates a cost-effective method using uniform 13C, 15N labeling of mid-size eukaryotic membrane proteins in Pichia pastoris, using 13C-methanol as a main carbon source. The produced samples are homogeneous, functional, stable, and yield high-resolution solid-state NMR spectra suitable for structural studies.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
