prepGEM Universal
prep GEM Universal is a widely-used, adaptable reagent ideal for rapid DNA extractions from life science samples such as blood, saliva, cell culture, and tissue (including ear punches, tails, and insect). The heat-driven extraction is done in a single-tube with no harsh chemicals or additional steps so there is no loss of DNA, enabling extractions from very low sample volume. The kit is perfect for CRISPR/Cas9 gene editing a…
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It is a great product.
Virus
It is easy to use.
Review Date: 21 Oct 2020 | MicroGEM
prepGEM Universal is MicroGEM’s comprehensive solution for DNA extraction from blood, saliva, and tissues. It is also perfectly suited for extracting DNA from cell cultures. All within a single tube, this method ensures no loss of DNA, and as a result, it excels with low cell number extractions.
prepGEM Universal utilizes a thermophilic proteinase activated with a simple temperature change. The whole process is easily automated in a single tube using a standard thermocycler.
- Stream-lined approach provides DNA in under 15 minutes
- Inhibitor-free solutions mean no harsh chemical washes or multiple steps
- Closed-tube extraction protects the integrity of the sample and preserves precious samples
- Simplified, hands-off workflow frees up technician time, reduces lab costs, and significantly reduces hazardous waste and plastic consummables
This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing and is also suitable for:
- STR
- PCR and qPCR
- Whole genome sequencing
- Amplicon sequencing
Brochures
Enabling next-generation molecular techniques & rapid nucleic acid extractions
This brochure demonstrates MicroGEM's aim to advance scientific discovery by developing devices that will allow anyone, whether researcher or layperson, to tackle complex biological problems such as measuring gene expression, detecting specific viruses, or authenticating food attributes or components.
Efficient DNA Extractions for the CRISPR Toolkit
CRISPR genotyping is a necessary step in the ever-growing area of CRISPR-Cas9 genome modification to identify which cell-lines have the desired modification. This process can often be laborious and, with the inherent loss of DNA from traditional DNA extraction methods, long proliferation times are required to ensure an adequate number of cells and therefore adequate quantities of DNA. The MicroGEM rapid protocol, with its purely enzymatic approach using a thermostable proteinase and buffer system, aims to allow researchers to shorten proliferation time, limit extraction time and process a large number of samples simultaneously to streamline the CRISPR genotyping workflow to a few, simple, efficient steps.
Processing forensic samples using a single-tube approach
Reducing agents, such as DTT, have been used to extract DNA from sperm cells in forensic sexual assault samples for many years. These reducing agents require harsh detergents and denaturants in the lysis buffer to reduce disulfide bonds. The resulting lysate contains reagents and proteins that must be removed via purification steps prior to qPCR and/or STR amplification. In this webinar, Jeff Hickey from MicroGEM, demonstrates how a single-tube protocol can help efficiently process sexual assault samples, including Y screening and differential extractions.
Addressing the challenges of DNA extraction from insects
In this webinar, discover a simple protocol for extracting DNA from insects using a single tube, without ionic detergents, magnetic beads, columns or centrifugation. The method allows efficient extraction of DNA from insects while maximizing yields and minimizing waste.
