Processing forensic samples using a single-tube approach
29 Apr 2020
Reducing agents, such as DTT, have been used to extract DNA from sperm cells in forensic sexual assault samples for many years. These reducing agents require harsh detergents and denaturants in the lysis buffer to reduce disulfide bonds. The resulting lysate contains reagents and proteins that must be removed via purification steps prior to qPCR and/or STR amplification. In this webinar, Jeff Hickey from MicroGEM, demonstrates how a single-tube protocol can help efficiently process sexual assault samples, including Y screening and differential extractions.
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MicroGEMforensic GEM Sperm is a rapid kit for extracting DNA from tough sperm heads. Using temperature controls and single-tube process, this kit requires no reducing agents, such as SDS, mercaptoethanol or DTT, that inhibit qPCR and STR analysis, As a result, DNA is preserved, processing time is quick, and opportunities for contamination are minimized making this the perfect approach for differential extractions and y-screening.
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MicroGEMprep GEM Universal is a widely-used, adaptable reagent ideal for rapid DNA extractions from life science samples such as blood, saliva, cell culture, and tissue (including ear punches, tails, and insect). The heat-driven extraction is done in a single-tube with no harsh chemicals or additional steps so there is no loss of DNA, enabling extractions from very low sample volume. The kit is perfect for CRISPR/Cas9 gene editing applications.
