Efficient DNA Extractions for the CRISPR Toolkit
22 Jun 2020CRISPR genotyping is a necessary step in the ever-growing area of CRISPR-Cas9 genome modification to identify which cell-lines have the desired modification. This process can often be laborious and, with the inherent loss of DNA from traditional DNA extraction methods, long proliferation times are required to ensure an adequate number of cells and therefore adequate quantities of DNA. The MicroGEM rapid protocol, with its purely enzymatic approach using a thermostable proteinase and buffer system, aims to allow researchers to shorten proliferation time, limit extraction time and process a large number of samples simultaneously to streamline the CRISPR genotyping workflow to a few, simple, efficient steps.
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prepGEM Bacteria
MicroGEMprep GEM Bacteria is ideal for processing Gram-positive bacteria, Gram-negative bacteria, protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water. The heat-driven extraction is done in a single-tube with no harsh chemicals or additional steps so there is no loss of DNA, enabling extractions from very low sample volume.
prepGEM Universal
MicroGEMprep GEM Universal is a widely-used, adaptable reagent ideal for rapid DNA extractions from life science samples such as blood, saliva, cell culture, and tissue (including ear punches, tails, and insect). The heat-driven extraction is done in a single-tube with no harsh chemicals or additional steps so there is no loss of DNA, enabling extractions from very low sample volume. The kit is perfect for CRISPR/Cas9 gene editing applications.