prepGEM Bacteria
prep GEM Bacteria is ideal for processing Gram-positive bacteria, Gram-negative bacteria, protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water. The heat-driven extraction is done in a single-tube with no harsh chemicals or additional steps so there is no loss of DNA, enabling extractions from very low sample volume.
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MicroGEM’s Bacteria kit provides a simplified approach to studying a wide range of microbial cultures. Every aspect of the MicroGEM method ensures the samples are processed quickly and gently. Minimal sample prep, closed tube processing, and gentle lysis ensure that cross contamination and DNA fragmentation are drastically reduced, making MicroGEM’s heat-driven, single-tube extraction process streamlined while preserving the integrity of low abundance species.
The process utilizes a thermophilic proteinase activated with a simple temperature change. The whole process is easily automated in a single tube using a standard thermocycler.
- Novel temperature controlled enzymatic lysis using a thermophilic proteinase in combination with mesophilic Lysozyme
- Rapid workflow- 1/4th the time taken by other methods
- Easily automated for high throughput
- Closed system- no cross contamination and protection against pathogens
- Gentle lysis with no transfer steps- preserves DNA and improves community representation in metagenomic samples
- Minimal handling and pipetting of samples- DNA is exceptionally intact and high molecular weight – perfect for nanopore sequencing
- Can be easily adapted to different sample types and workflows
Brochures
Enabling next-generation molecular techniques & rapid nucleic acid extractions
This brochure demonstrates MicroGEM's aim to advance scientific discovery by developing devices that will allow anyone, whether researcher or layperson, to tackle complex biological problems such as measuring gene expression, detecting specific viruses, or authenticating food attributes or components.
Efficient DNA Extractions for the CRISPR Toolkit
CRISPR genotyping is a necessary step in the ever-growing area of CRISPR-Cas9 genome modification to identify which cell-lines have the desired modification. This process can often be laborious and, with the inherent loss of DNA from traditional DNA extraction methods, long proliferation times are required to ensure an adequate number of cells and therefore adequate quantities of DNA. The MicroGEM rapid protocol, with its purely enzymatic approach using a thermostable proteinase and buffer system, aims to allow researchers to shorten proliferation time, limit extraction time and process a large number of samples simultaneously to streamline the CRISPR genotyping workflow to a few, simple, efficient steps.
