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PMA (Propidium Monoazide)
A photo-reactive DNA-binding dye typically used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi.
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PMA (propidium monoazide) is a photo-reactive DNA-binding dye used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi. PMA is available as a 1 mg lyophilized solid or 20 mM solution in water. Also try PMAxx™ (40069), a superior alternative to PMA.
- Selectively detect viable cells using qPCR
- Validated in hundreds of publications
- Dead cell specific dye, binds to DNA
- Covalently attaches to DNA after photoactivation
- Available as 1 mg lyophilized solid or 20 mM solution in water
- λAbs = 464 nm (before photolysis); λAbs/λEm= ~510/~610 nm (with DNA/RNA, after photolysis)
