Phree Phospholipid Removal Solutions

Sample preparation is crucial in achieving desired LC or GC analytical results. Sample matrix effects can result in an array of interferences which can lead to poor chromatography as well as instrumentation drawbacks, hindering your approach and goal for the analysis.

In this user guide, Phenomenex Inc provides all the information you need to choose and use the best sample preparation technique for your work, whether it's filtration, QuEChERS, SLE, PPT, PLR, or SPE. Download your free copy below to read recommendations for your industry, general methods, protocols for easy method development, and much more.

Whole blood is a common matrix for toxicology testing, yet it can be very complicated to clean up. Using Phree Phospholipid Removal as a pass through with an easy protein precipitation, the whole blood matrix is efficiently cleaned up while still yielding high recoveries and low variation before analysis on LC MS/MS using a Kinetex Biphenyl LC column.

Non-psychoactive cannabinoids are a promising avenue for medical research with potential therapeutic uses, however due to their chemistry phospholipid contamination can be an issue for chromatographic separation. This application note presents an LC-MS/MS method with an extra phosphate-removal step for reduced ion suppression/matrix effects.

This application note aims to help you improve your methods for more effective and quicker bioanalytical sample preparation.

The world is changing and we need to widen our Environmental Scope. Although analytical techniques and established regulated methods are not easily changed; more complex targets, larger lists, and a variety of sample matrices create a daily challenge for environmental chemists. By using the latest technologies and techniques in HPLC/ UHPLC and Sample Preparation, laboratories and chemists are able to develop better approaches to make their work Faster, Cheaper and Easier. Phenomenex is committed to supporting the industry through a comprehensive product portfolio of Sample Preparation, HPLC/UHPLC, and LC-MS, columns and accessories, along with application and method development services to meet industry needs.

This application note discusses sample preparation techniques for a variety of clinical samples for simplified liquid extractions (SLE) and solid phase extraction (SPE).

In this application note, protein precipitation is compared to a phospholipid removal product, Phree Phospholipid Removal Plates, to assess the clean up capabilities of each technique. LC/MS/MS analysis of the phospholipids present each sample indicates that Phree Phospholipid Removal Plates result in significantly cleaner samples, reduced ion suppression, and extended HPLC/UHPLC column lifetime. Phree Phospholipid Removal is a simple procedure similar to protein precipitation, requires no method development and can be automated to provide higher throughput.

This application note describes how one quick method that can be used to remove proteins that clog up HPLC columns, eliminate phospholipids that cause ion suppression and increase column lifetime. This method can be used for acids, bases and neutrals, saving time spent on method development. Tips on selecting organic solvents, loading capacities and ordering products are also provided.

Phospholipids left in a sample after protein precipitation can decrease your HPLC/UHPLC column lifetime and reduce analyte sensitivity due to build-up on your column and can also increase mass spec maintenance. This application note demonstrates how a very simple protocol using Phree Phospholipid removal plates can remove almost all phospholipid to produce cleaner samples and provides ordering information.

General Unknown Screening (GUS) is the screening of many compounds from a whole blood matrix, which is especially important in cases where an affected individual is incapacitated, unaware of what was taken, or deceased. This application note describes a universal sample preparation procedure for whole blood samples and a method for neutralising the undesirable effects of strong solvents to allow immediate chromatographic analyses. This is a simple and fast procedure that yields successful and reliable analyte identification.

In this webinar, Dr. Stephanie Marin, Global Clinical Market Development Manager at Phenomenex, will provide a comprehensive overview of polymeric mixed-mode solid phase extraction (SPE) fundamentals and the properties of polymeric hydrophobic interaction (reversed-phase) SPE sorbents. Attendees will learn how to choose the appropriate SPE phase for their specific compounds of interest, along with an explanation of the ionic functional groups that provide the mixed-mode functionalities, including both weak and strong cation and anion exchange.

Dr. Marin will also delve into the initial extraction conditions required to initiate method development for each type of SPE sorbent, as well as specialized SPE products tailored for drug testing, phospholipid removal, and microelution SPE. This webinar will include numerous clinical research applications to exemplify these concepts.

Key learning objectives

• Discover how to differentiate different polymeric mixed-mode SPE sorbents
• Explore starting conditions for each sorbent type
• Learn how to choose the best SPE sorbent for different analytes
• Understand how to use an SPE method development plate

Who should attend?

• Scientists doing SPE method development
• Labratorians who want to increase their knowledge of polymeric mixed-mode SPE
• Lab managers who want to implement SPE methods in their laboratories

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Phenomenex’s sample preparation product manager shares his expert advice on preparing challenging biological sample matrices for LC-MS/MS