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phi29 DNA Polymerase

phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5´ proofreading exonuclease activity. Source: An E. coli strain that carries the phi29 DNA Polymerase gene from bacteriophage phi29 Applications: •Replication requiring a high degree of strand displacement and/…

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New England Biolabs Inc.

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Average Rating 4.7

|1Scientists have reviewed this product

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Consistent results with high concentration DNA obtained for lower cost

 

Average Rating 4.7

Application Area:

HTS DNA amplification for Cell-Free Expression

This product is the definition of value for money! You can trust that it will do the job, and when used with appropriate buffers and protocol, it costs significantly less than RCA/phi29 amplification kits. It was also very consistent when used for different starting material (pure plasmid or E.coli colony) and for different plasmid constructs. I haven't needed the after sales much, but they were always very quick to deliver.

Review Date: 13 Aug 2023 | New England Biolabs Inc.

phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5´ proofreading exonuclease activity.

Source:
An E. coli strain that carries the phi29 DNA Polymerase gene from bacteriophage phi29

Applications:

•Replication requiring a high degree of strand displacement and/or processive synthesis
•High fidelity replication at moderate temperatures

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