Metabolomics
Isotope-labeled Standards and Mixtures / Kits for Metabolomics Research
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Metabolomics is an increasingly important and growing area of research. The use of stable isotope-labeled standards, in combination with mass spectrometry, allows researchers to identify and quantify metabolites in a given biological sample. This information is used to better understand the physiology of an organism, its response to a drug, a change in its environment, and/or other external stimuli. It is also used to unravel disease mechanisms, assess putative biomarkers, and to monitor metabolic pathway activities (i.e., flux). To aid such research initiatives, CIL offers a library of isotopically labeled metabolite standards (span a multitude of metabolic classes and pathways) that are available individually and/or as multi-component mixtures and kits. The mixes are formulated neat and/or as solutions and are readily available for immediate use. In addition to the packaged mix(es), the kits include a user manual, which outline general procedures and processing examples for user reference.
Targeted MRM screening of U-13C lipid yeast extracts for robust lipidomics applications
Fatty acids (FAs) and lipids are important biological compounds that are essential to the regulation and control of cellular functions (e.g., membrane structure, signal transduction, apoptosis) and metabolic pathways (e.g., fatty acid oxidation, ketone body synthesis, lipolysis). These biomolecules are also functionally important to an organism’s energy production and storage.
In this application note, Cambridge Isotope Laboratories qualifies a condensed panel of stable isotope-labeled FAs/lipids in U-13C crude lipid yeast extracts on robust, high-throughput, analytical platforms.
Resource details:
- Resource type: Application note
- Page count: 8
- Read time: 12 mins
Standardizing quantitative metabolomics analyses through the QReSS Kit
One of the incessant challenges in mass spectrometry (MS)-based “omics” is the qualification of methods and instrument platforms. Required are materials that can be used to routinely test platform performance over time and to test assay effectiveness throughout all phases of a work list. Such evaluations are necessary as it provides an indicator to the effectiveness of an analytical method and platform. Errors or deficiencies observed in the performance would then cue corrective action. This could help save precious samples and experimental resources, while also extend column lifetimes.
Resource details:
- Resource type: Application note
- Page count: 10
- Read time: 15 mins
Tracing lipid disposition in vivo using stable isotope-labeled fatty acids and mass spectrometry
Dysfunctions of lipid metabolism contribute to a variety of diseases including, among others, atherosclerosis, hypertriglyceridemia and type 2 diabetes. Stable isotopes have been used to study several aspects of lipid metabolism including the synthesis and disposition of cholesterol,1,2 phospholipids3 and VLDL triglycerides. In this application note, Cambridge Isotope Laboratories highlight some of the advantages and experimental considerations for using stable isotope-labeled fatty acids as substrates to study lipid metabolism in vivo in mice.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
Metabolic isotopic analysis reveals mitochondrial loss of function in pRb-deficient cells in vivo
Metabolic isotopic analysis (MIA) utilizes stable isotope-enriched substrates and mass spectrometry to study metabolism in living cells and tissue. Allowing the determination of metabolic pathways and flux in living cells or tissue possible, MIA has become an integrated platform in oncology research. In this application note, Cambridge Isotope Laboratories presents an example of MIA for studying metabolism in mice and cultured human cells.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
