Lunatic

In this application note, Unchained Labs describes how the Lunatic performs quantitative spot checks by measuring the full UV/Vis spectrum and independently quantifying the plasma-relevant components: hemoglobin, bilirubin, and protein, along with sample turbidity profiles. This application note highlights Lunatic as a device that promises high-throughput analysis and quantification of plasma.

Analyzing quantifications one by one while having to dilute highly concentrated samples is laborious and time consuming. The Lunatic platform overcomes these hurdles with a dynamic range from 0.02–200 mg/mL (mAb) without ever needing to dilute. The Lunatic, Big or Little, is your step up to the next-generation UV/Vis reader allowing you to measure up to 96 samples in 5 minutes using only 2 μL of an undiluted sample.

The conformational, chemical and colloidal stability of a protein are strongly influenced by the buffer solution. Altering buffer salts, pH, ionic strength, excipients and surfactants may increase or decrease the stability of a molecule. The process of screening formulation buffers to optimize stability is a time consuming process. The time required to perform buffer exchange is often a limiting factor in biologics development. Junior with buffer exchange removes the buffer exchange bottleneck by automating buffer exchange and sample concentration, which reduces required hands-on time and increases the number of samples that can be prepared in a day.

Wouldn’t it be mind-blowing if you could accurately measure your highly concentrated biologics in a dilution-free, accurate and high-throughput way? The Lunatic platform tackles these issues with confidence; the platform covers a dynamic range from 0.02–150 mg/mL (mAb) and applies a unique turbidity correction for more accurate quantification.

This application note describes how to use the colorimetric assay applications on the Lunatic systems. Protein quantification is often done by measuring absorbance at 280 nm, results however might be influenced by buffer or source constituents. An alternative to A280 quantification are colorimetric protein assays. Reagent(s) and proteins are mixed, producing a color change which is a measure for the amount of protein present. Unknown sample concentrations are calculated using a measured standard curve.

This application note describes how to use the DNA mammalian application on the Lunatic systems. This Unmix application is used to analyze the UV/ Vis spectral shape of the sample to isolate the fraction of the molecule of interest from co-absorbing entities contributing to the total UV/Vis absorption spectrum. This applies to genomic DNA (gDNA) from different mammalian sample sources (human or animal blood, saliva, tissue or cell line...) that are extracted using different extraction methods or commercial kits for DNA isolation.

In this note, Unchained Labs describe how to use the Protein labeled application on the Lunatic systems. This application determines the concentration of the protein, the amount of dye present in labeled protein samples, and furthermore calculates the degree of labeling (DoL)

The Lunatic enables quantification of up to 96 micro-volumes of protein, DNA, and RNA. With the add-on lunatic software features, described in this application note, the lunatic offers an effective data management platform for its users, and enables simple electronic record keeping.

This application note describes the use the RNA Cy3/ Cy5 and ssDNA Cy3/Cy5 applications on the Lunatic systems. These Unmix applications are used to analyze the UV/Vis spectral shape of the sample to isolate the fraction of the molecule of interest from co-absorbing entities contributing to the total UV/Vis absorption spectrum. These Cy3/Cy5 Unmix apps isolate the RNA or ssDNA profile as well as specific Cy3 and Cy5 profiles from the measured UV/Vis spectrum, thereby distinguishing them from other absorbing sample contaminants, determining both the concentration of the nucleic acids as well as the amount of dye present, and calculating the degree of labeling (DoL).

This application note describes how to use the Total Nucleic Acids application on the Lunatic systems. This Unmix application is used to analyze the UV/Vis spectral shape of the sample to isolate the fraction of the molecule of interest from co-absorbing entities contributing to the total UV/Vis absorption spectrum. Accurate quantification of the molecule of interest is established using its isolated spectrum fraction. This application does not distinguish RNA from DNA, but rather isolates the total nucleic acid concentration in RNA extractions from unknown sources (bacterial, yeast, plant, plasmid, etc.).

Tuesday, February 24 at 16:00 GMT | 17:00 CET | 11:00 EST | 08:00 PST

Next-generation sequencing (NGS) has opened new frontiers in disease discovery, diagnosis, and treatment, but biobanking and reliable sequencing demands consistent nucleic acid concentrations and quality. Fluorescent dye-based quantification is costly, time consuming, error-prone, and says nothing about purity. As biospecimen throughput increases, so does the need for fast, accurate low-volume quantification and QC.

Lunatic combines full-spectrum UV/Vis absorbance with a microfluidic plate to deliver one-step DNA or RNA purity and concentration from 96 samples in 10 minutes or less, without dyes or dilutions. For true high-throughput workflows, Lunatic integrates seamlessly with liquid handlers and plate loaders and always uses 2 µL per sample.

During this SelectScience® webinar, you will learn how Lunatic quantifies DNA and RNA with 2% accuracy and 1% precision, and see direct comparisons to fluorescence assays. You will also discover how full-spectrum UV/Vis with Unmix enables rapid purity assessment and decision-making. Plus, we will highlight real-world biobank applications for DNA quantification, normalization, and QC.

Key learning objectives:

• Learn how Lunatic combines UV/Vis absorbance measurements with a low volume 96-well plate to provide quick and easy reagent-free, dilution-free, and standard-free genomics quantification
• Understand the limitations of fluorescence-based quantification for NGS workflows
• Explore how to use Unmix to identify and quantify common contaminants
• See how biobanks and high-throughput labs use Lunatic for DNA quantification, normalization, and QC

Who should attend?

This webinar is ideal for genomics-focused scientists/R&D scientists in biotech and pharma, biologists, and geneticists.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Webinar details

• Cost: Free to attend
• Location: Online
• Duration: 60 minutes

Registration is required to secure your place. If you register but cannot attend live, you will receive a link to the on‑demand recording once it becomes available.

Watch this on-demand webinar to learn how to take your nucleic acid quantification to the next level of speed, accuracy, and throughput

Free up your time in the lab for the work that really matters

Watch this on-demand expert webinar exploring how solutions from Unchained Labs are designed to streamline protein quantification and characterization

Register for this expert webinar to find out how solutions from Unchained Labs can help improve your protein quantification and analysis