Invitrogen™ Click-iT™ Plus EdU Alexa Fluor™ 350 Flow Cytometry Assay Kit
The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed cova…
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The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to Alexa Fluor dyes. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population. For this kit, newly synthesized DNA is analyzed using the 350 nm laser of the flow cytometer. The Click-iT Plus formulation is compatible with standard fluorophores, including R-PE and R-PE tandems, as well as fluorescent proteins.
Features
- Accurate--superior results compared to BrdU Assays
- Fast--results in as little as 90 minutes
- Economical--more assays per kit
The Click-iT Plus EdU label is performed using much milder conditions than traditional BrdU assays. The EdU assay conditions are also compatible with cell cycle dyes and multiplexing using antibodies against surface and cellular markers.
Imaging protocol handbook
Thermo Fisher Scientific presents a trusted collection of imaging protocols, that includes step-by-step instructions for common imaging protocols, including tips for a successful experiment, and lists of needed supplies. Invitrogen™ reagents are recognized globally as key components for publication-quality cellular images and analysis. Thermo Fisher offers cellular structure and functional probes, labels, and counterstains, as well as tools for achieving stunning cellular imagery. Understand how technologies, including Invitrogen™ solutions, may be used to obtain useful cell images, such as: cell proliferation; cell viability; cell structure; live-cell assays; immunolabeling; and cell painting.
