Infinite F Nano+

AlphaLISA is a homogeneous, no-wash alternative to conventional ELISAs based on PerkinElmer’s bead-based Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology. AlphaLISA assays can be set up as sandwich or competitive immunoassays to detect and quantify molecules of interest in biological samples. AlphaLISA assays are less prone to signal interference at wavelengths of <600 nm and the use of dedicated AlphaLISA optics permits the analysis of target molecules in blood and serum by drastically reducing the effect of hemoglobin within a sample. This application note describes the implementation of the AlphaLISA technology onto the Infinite F200 PRO, and describes the use of the reader for the detection of human immunoglobulin G (IgG) using PerkinElmer’s AlphaLISA Human IgG Assay Kit.

Tecan developed an AlphaScreen® module for its popular Infinite® F200 PRO multimode reader which can provide customers with a cost-effective solution for AlphaScreen and AlphaLISA® assays. This application note describes the successful implementation of the Alpha technology onto the Infinite F200 PRO and summarizes the performance of the instrument with regard to measurement sensitivity, uniformity and typical reading times.

Growth analysis of micro-organisms is a fundamental part of many research areas yet can be highly time consuming and labor-intensive. Tecan have designed the Gas Control Module in conjunction with the Infinite 200 Pro Reader which allows walk-away growth monitoring through the controlled measurement chamber which provides all essential growth conditions, eliminating the need for transfer between incubators and readers and manual intervention. This application note describes the use of these systems in the analysis of growth of the human pathogen and class-I carcinogen, Helicobacter pylori, over a 28 hour period.

The Infinite 200 PRO is a user-friendly and affordable multimode reader, designed to cater for the needs of today’s applications. Providing a full range of leading detection methods in one easy-to-use modular instrument, multimode microplate readers (MMRs) are commonly equipped with photomultiplier tubes (PMTs) which are extremely sensitive detectors of light in the ultraviolet, visible and near-infrared ranges of the electromagnetic spectrum. This application note compares luminescence measurements using the Infinite M200 PRO with Photomultiplier Tube-based multimode readers.

The most widely established technology for UV-Vis absorbance measurement is a monochromator-based microplate reader. Tecan’s Infinite M200 PRO is a user-friendly and affordable multimode reader, designed to cater for the needs of today’s applications, providing a full range of leading detection methods in one easy-to-use modular instrument with Quad4 monochromator™. This application note compares the Infinite M200 PRO with a multimode reader using photodiode array technology on their dynamic measurement range, OD linearity in the visible and the UV range and measurement reproducibility.

Assessment of cellular viability markers in cell-based applications is mandatory for laboratories working with in vitro systems. Life Technologies offers an enhanced version of the commonly available resazurin-based assays, the PrestoBlue® Cell Viability Reagent. This application note describes the evaluation of Life Technologies’ PrestoBlue® Cell Viability Reagent using the Infinite M200 PRO and the Infinite M1000 PRO multimode microplate readers.

Reactive oxygen species (ROS) are generated as natural by-products of the cellular metabolism. They are involved in various biological processes, functioning as important signal mediators. However, excess intracellular levels of ROS may result in cell and tissue damage, and are associated with degenerative diseases, most notably cancer. A well-established and reliable method to determine the antioxidant capacity of a substance is the oxygen radical absorbance capacity (ORAC) assay. This application note describes the use of the Infinite® F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples.

This application note describes the capability of Tecan’s Infinite® 200 PRO multimode reader to regulate the CO2 partial pressure inside its measurement chamber. The investigation of biological processes requires time-dependent analysis of cellular signals, from several hours up to several days. However, long-term studies with living eukaryotic cells can only be performed for a limited time inside common microplate readers.

Fluorescence measurements in general depend on various parameters which can all be optimized to achieve better results in terms of signal intensity and quality. This application note describes the relevant instrument parameters to acquire optimal fluorescence excitation and emission spectra on the Infinite M200 PRO and Infinite M1000 PRO.

Quantification of small molecules in biological samples (such as serum, plasma, urine or blood) by LC-MS is generally only performed after a sample clean-up step. This is due to the presence of specific matrix constituents – such as proteins and phospholipids – which might negatively affect the analysis. The Tecan AC Extraction Plate simplifies sample preparation by efficiently removing most matrix components – such as proteins and salts – without any precipitation, filtration, centrifugation and evaporation steps.