Products & ReviewLife Sciences

Incucyte® SX5 Live-Cell Analysis System

Sartorius GroupView all modelsAvailable: Worldwide

Leading the Way With Living Cells, our most advanced application offering.

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Average Rating 5.0

|2Scientists have reviewed this product

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Great results with unbiassed reproducible and reliable data.


Average Rating 5.0

Application Area:

Life Sciences - Neuroscience, microbiology, cell biology

The product is really simple to set up and use. It's becoming a work horse for our lab. It gives great replicable data and is providing us with very insightful real time analysis of different treatment of live human primary cells in culture. This is enabling us to screen for various putative novel drugs and help us better understand signaling cascades downstream after addition of any biological agent.

Review Date: 3 Mar 2021 | Sartorius Group

Superior results - our scientists' favourite technology in the lab!


Average Rating 5.0

Application Area:

Live cell imaging and analysis

The Incucyte SX5 is an easy-to-use live-cell imaging technology, saving our scientists time in both experimental set-up and monitoring whilst providing exceptional, previously unobtainable readouts. Protocols for a wide range of lab-based assays are freely available and easy to follow. Many of our scientists have already switched many of their traditional lab-based assays to use the Incucyte technology instead. The ability to monitor your cells in real-time remotely is a hugely attractive feature to our scientists especially during this pandemic. The technology provides user-friendly built-in software to easily evaluate your experimental data offering a range of options to present your data graphically, via FACS plots or in movies – all of which are easily transferable to other software for presentation or publication purposes. Both the sales reps and application specialists were extremely helpful during our purchase and training and continue to provide support/advice to our scientists for their individual experimental needs. The fact that our Incucyte has been in non-stop use since our purchase is testament to our scientists' satisfaction with this technology and we would not hesitate in recommending this technology to other labs.

Review Date: 23 Feb 2021 | Sartorius Group

The Incucyte® SX5 is our most advanced application offering.

Study complex immune-tumor cell interactions, synaptic activity in neuronal co-cultures, metabolism in cancer cells, and much more — with a single platform.
See more information in every sample with the new Incucyte® SX5 featuring patent-pending optics. Do more with up to 5 different fluorescent channels specifically designed for live-cell analysis.
Enjoy more colors. More reagents. More purpose-built software for more applications. Derive deeper, physiologically relevant information about your cells.

Never miss powerful insights again.

Conventional approaches to cell analysis only capture a single time point, enabling only single-point and end-point measurements, and cells are perturbed or destroyed as part of the assay process.

The Incucyte® SX5 Live-Cell Analysis System automatically acquires and analyzes images around the clock, enabling you to derive deeper and more physiologically relevant information about your cells, plus real-time kinetic data — without ever removing your cells from the incubator. Change can happen in an instant. See what your cells are doing and when they do it.

The Incucyte® SX5 instrument, proprietary assays and reagents provide you with the ability to gain new insights into biological processes via real-time, quantitative analysis of live cells.

Furthermore, the Incucyte® SX5 accommodates multiple users and applications seamlessly and combines information-rich, image-based analysis with the convenience and throughput of microplate assays. Incucyte® technology is featured in over 3,000 peer-reviewed publications in journals.

Key features

  • Up to five different fluorescence channels, up to three at a time in a single experiment
  • New 3-color optical module includes a long wavelength, low phototoxicity NIR channel and optimized reagents for turnkey applications
  • Includes 4x, 10x, and 20x objectives on an automated turret
  • Supports 3 interchangeable vessel trays and over 600 vessels, up to 6 microplates in parallel
  • Seamless multi-user support via remote, networked access and unlimited, free licenses


  • Monitor metabolism in cancer cells
  • Complex immune-tumor cell interactions
  • Synaptic activity in neuronal co-cultures
  • Proliferation (confluence and cell counts)
  • Apoptosis (caspase 3/7 for live-cell imaging)
  • Cytotoxicity
  • Dilution cloning (whole-well imaging)
  • Migration / Invasion
  • Stem cell monitoring and reprogramming
  • 3D-Spheroids Angiogenesis
  • Neurite outgrowth and dynamics
  • Neuronal Activity
  • Reporter gene expression
  • Viral studies
  • Immune response – Immune cell killing
  • Antibody Internailization
  • NETosis
  • Phagocytosis
  • Immune cell clustering
  • Immunocytochmistry
  • Cell-by-Cell Analysis
  • Cell culture (& QC)

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