Celloger® Pro, Automated Live Cell Imaging System from Curiosis

In this application note, CURIOSIS explores how the physical state of solid tumor cells affects NK cell-mediated cytotoxicity using Celloger® Pro, an automated live-cell imaging system. With its high-resolution, time-lapse imaging capabilities, Celloger® Pro enabled real-time visualization of tumor-immune interactions, providing a powerful tool for investigating cytotoxic mechanisms and advancing immunotherapeutic strategies.

In this application note, CURIOSIS investigates NK cell-mediated cytotoxicity in a U-2OS spheroid model using real-time imaging. This was achieved using the Celloger® Pro live-cell imaging system, which enabled continuous, non-invasive visualization of immune-tumor interactions. These findings highlight the value of real-time imaging in assessing immune effector functions and support the use of spheroid-based assays as a reliable platform for immunotherapy research.

In this application note, CURIOSIS demonstrates a method to analyze natural killer (NK) cell cytotoxicity in real time with improved accuracy and efficiency. This method uses the automated live cell imaging system Celloger® Pro along with optimized staining reagents.

This application note presents a method to analyze NK cell cytotoxicity in real time with improved accuracy and efficiency.

Adipocytes serve as crucial energy reservoirs, efficiently converting surplus calories into fat for future energy needs. However, excessive fat accumulation can lead to obesity, a condition associated with various health problems in our modern society. Beyond their role in metabolism, adipocytes also influence immune responses and reproductive functions through hormone utilization such as leptin and adiponectin. Therefore, assessing the degree of adipogenesis at different time points is crucial. The use of automated live-cell imaging equipment, such as the Celloger® Pro, makes it convenient to monitor and measure adipogenesis. Curiosis introduces methods for assessing adipogenesis during the differentiation process using the widely studied preadipocyte cell line, 3T3-L1.

Cytokinesis is the final stage in cell division, during which the cytoplasm of one cell is physically divided into two separate cells. Actin filaments play a crucial role in supporting cell structure and facilitating division. Just before cell separation, actin filaments constrict the cell membrane, leading to the formation of two daughter cells. Cytochalasin B, a compound widely used in cell division and movement research, significantly affects the structure and dynamics of actin filaments. It primarily hinders cytokinesis by blocking the formation of contractile microfilaments. Additionally, previous studies have shown that cytochalasin B can lead to incomplete cell division, resulting in the formation of multinucleated cells. Curiosis demonstrates dynamic changes in cell structure upon cytochalasin B treatment using the Celloger® Pro live cell imaging system. By enabling real-time cell monitoring inside the incubator, it allows for seamless observation and tracking of cellular dynamics without disrupting the natural growth environment.

Mitochondria serve as the primary organelles responsible for generating energy within cells. In particular, mitochondrial membrane potential (MMP) is considered a key indicator of mitochondrial function, as it is vital for adenosine triphosphate (ATP) production. Hence, MMP measurement has long been used as a marker for evaluating apoptosis, cell viability, and overall cellular health. In healthy cells with normal MMP, JC-10 accumulates within the mitochondria, forming aggregates that emit red fluorescent wavelengths. In unhealthy cells with declined MMP, such as apoptotic cells, JC-10 scatters across the cytoplasm and converts into monomers, emitting green fluorescent wavelengths. Curiosis introduces a procedure for evaluating mitochondrial membrane potential using Celloger® Pro live cell imaging system. The system's dual fluorescence and bright-field microscopy enable simultaneous visualization of multiple markers, while the multi-point time-lapse imaging captures dynamic cellular events across different locations.

In this application note, CURIOSIS presents the Celloger® Pro, a cutting-edge live cell imaging system, used in a study to investigate the effects of the anticancer drug Staurosporine on 3D spheroids made from HEK293-GFP stable cells. The results showcase Celloger Pro's capability to dynamically capture and quantify cellular responses to drug treatment in a three-dimensional context, contributing to advancements in drug safety and efficacy evaluations for early-stage drug discovery and development.

In this video, CURIOSIS demonstrates how the Celloger® Pro can be used to observe the actin depolymerization process. HeLa cells stably expressing tdTomato-tagged actin were treated with cytochalasin B at low (1.25 μM) and high (10 μM) concentrations. Time-lapse images were taken at 1-hour intervals over 48 hours using a Celloger® Pro with a 10X objective.

In this video, CURIOSIS demonstrates how the Celloger® Pro can be used to studying NK cell interactions with target cells. To observe these interactions, U-2OS cells (targets) were stained with CellTracker Green CMFDA (green fluorescence) and co-cultured with NK-92 cells (effectors) at effector-to-target ratios of 5:1 and 20:1. Time-lapse imaging began immediately after staining with 4 µM EthD-1 (red fluorescence) and was performed every hour for 24 hours using a Celloger® Pro with a 4X objective lens.

In this video, CURIOSIS demonstrates how the Celloger® Pro can be used to observe the drug effect on spheroids in real-time. Spheroids were made from HEK293 cells that stably express GFP. Staurosporine and EthD-1 dead cell staining dye were applied, and the images were captured at 30-minute intervals over 24 hours using a Celloger® Pro with a 2X objective.

In this video, CURIOSIS demonstrates how the Celloger® Pro can be used to monitor transfection efficiency in real-time. In AGS cell line, cell type was stained with CMFDA dye (green fluorescence) and then transfected with the tdTomato-Lifeact gene (red fluorescence). Seven hours post-transfection, time-lapse imaging was performed every 90 minutes for 65 hours using the Celloger® Pro with a 2X objective.

In this video, CURIOSIS demonstrates how the Celloger® Pro can be used to monitor the adipocyte differentiation process. To show this, 3T3-L1 cells were stained with LipiDye, and time-lapse images were taken at 1-hour intervals over 48 hours using a Celloger® Pro with a 10X objective. As the cells differentiate, an increase in the distribution of lipid droplets exhibiting green fluorescence can be observed over time.

In this video, CURIOSIS demonstrates the Celloger® Pro, an innovative live cell imaging system that redefines research capabilities, and how to utilize its analysis app.

In this video, CURIOSIS demonstrates the Celloger® Pro, an innovative live cell imaging system that redefines research capabilities. Its advanced features allow for seamless observation and tracking of cellular dynamics without disrupting the natural growth environment.

In this video, CURIOSIS demonstrates the Celloger® Pro, a live cell imaging solution for scientific exploration. The system's dual fluorescence and bright-field microscopy enable simultaneous visualization of multiple markers, while the multi-point time-lapse imaging captures dynamic cellular events across different locations. Celloger Pro’s user-friendly interface and intuitive tools make image acquisition and analysis a seamless experience. The system enables real-time cell monitoring inside the incubator, and seamless observation and tracking of cellular dynamics without disrupting the natural growth environment. Additionally, its ability to capture high-resolution images and to effortlessly create videos, eliminates the labor-intensive tasks associated with live cell imaging processes.

Expand horizons in life science research and discover the power of real-time cell monitoring