Visualizing NK cell-tumor interactions: A dynamic approach to spheroid-based tumor killing assays using Celloger Pro

In this application note, CURIOSIS investigates NK cell-mediated cytotoxicity in a U-2OS spheroid model using real-time imaging. This was achieved using the Celloger^® Pro live-cell imaging system, which enabled continuous, non-invasive visualization of immune-tumor interactions. These findings highlight the value of real-time imaging in assessing immune effector functions and support the use of spheroid-based assays as a reliable platform for immunotherapy research.