Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized
Preservative-free Annexin V conjugates are compatible with real-time staining of apoptotic cells for live cell imaging, fluorescence microscopy, or flow cytometry.
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CF® Dye Annexin V Conjugates can be used to stain the surface of apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.
- Azide-free for no-wash, real-time live cell imaging in culture medium
- Fast & simple detection of phosphatidylserine on apoptotic cells
- Choice of 11 CF® dye colors with superior brightness & photostability
- For real-time live cell imaging, fluorescence microscopy, or flow cytometry
Preservative Free for Real-Time, Live Cell Imaging
Annexin V conjugates typically are supplied as stock solutions with azide as a preservative for end-point staining assays in Annexin V binding buffer. Our azide-free CF® Dye Annexin V Conjugates are supplied as lyophilized solids with no azide or other preservatives that might be incompatible with live cell imaging. After reconstitution in buffer, the conjugates can be added to cell culture medium for no-wash, real-time live cell imaging. Our Mini Syringe Filters are convenient for small volume sterile filtration of azide-free Annexin V stock solutions or other aqueous solutions for use in cell culture.
Annexin V, CF®647 Conjugate, Azide-Free, Lyophilized #29003R-5ug
Annexin V, CF®555 Conjugate, Azide-Free, Lyophilized #29004R-5ug
Annexin V, CF®488A Conjugate, Azide-Free, Lyophilized #29005R-5ug
Annexin V, CF®633 Conjugate, Azide-Free, Lyophilized #29008R-5ug
Annexin V, CF®405M Conjugate, Azide-Free, Lyophilized #29009R-5ug
Annexin V, CF®568 Conjugate, Azide-Free, Lyophilized #29010R-5ug
Annexin V, CF®594 Conjugate, Azide-Free, Lyophilized #29011R-5ug
Annexin V, CF®350 Conjugate, Azide-Free, Lyophilized #29012R-5ug
Annexin V, CF®640R Conjugate, Azide-Free, Lyophilized #29014R-5ug
Annexin V, CF®660R Conjugate, Azide-Free, Lyophilized #29069R-5ug
Annexin V, CF®450 Conjugate, Azide-Free, Lyophilized #29083R-5ug
Annexin V, CF®583R Conjugate, Azide-Free, Lyophilized #29085R-5ug
