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Agilent BioTek Cytation 5 Cell Imaging Multimode Reader

Agilent TechnologiesAvailable: Worldwide

Agilent BioTek Cytation 5 is a uniquely integrated, configurable system that combines automated digital widefield microscopy with conventional multi-mode microplate detection to provide phenotypic cellular information and well-based quantitative data.

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Average Rating 4.6

|41Scientists have reviewed this product

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I love it and anyone who uses it does too


Average Rating 5.0

Application Area:


Easy to use, high throughput reader that continues to be an integral part of our lab

Review Date: 29 Mar 2022 | Agilent Technologies

Robust equipment, not extremely user friendly, but technical support is excellent.


Average Rating 4.0

Application Area:

Bright field and fluorescent cell analysis

I mostly use the instrument for cell count (bright field and fluorescence), virus infectivity evaluation, and identifying cytoplasmic and nuclear proteins. The instrument is robust and works well when using fluorescence. The bright-field cell count is less robust, but it works for some applications and needs some extra work to make it more accurate. Setting up a protocol for an automatic read of plates is not very simple, but the FAS are always available to give support to design or evaluate a new protocol.

Review Date: 15 Nov 2021 | Agilent Technologies

Great equipment and amazing tech support.


Average Rating 4.3

Application Area:

ICC and bright field cell count

The Cytation 5 is a powerful instrument that helped me to perform bright field cell count of attached cells and analysis of cell markers, such as PAX7, MHC and Desmin. It is not the most user friendly software around, especially because you have a lot of possibilities for cell analysis and creation of automated protocols, so it takes some time to become familiar with the equipment/software. That being said, the support offered by the Field Application Specialists is amazing. I used a lot of their help to create my protocols for automated cell analysis. The regular Tech support is also really good.

Review Date: 19 Jul 2021 | Agilent Technologies

Very good image quality, ideal for following individual cells!


Average Rating 3.7

Application Area:

Drug discovery

Cytation 5 is a fantastic cell image reader. It also gives very good image resolution.

Review Date: 7 Apr 2021 | Agilent Technologies

It is indeed a great product, can't live without it!


Average Rating 5.0

Application Area:

Oncology research

An excellent instrument that serves wide range of applications such as imaging and kinetics. It is a very valuable core instrument that serves multiple pharmacy labs at University of Toronto.

Review Date: 8 Feb 2021 | Agilent Technologies

Good system but needs key end user for imaging.


Average Rating 4.0

Application Area:

Cell imaging and plate reader

The cell imaging requires some finagling. Vibration can be an issue at higher objectives. The plate reader portion works like a charm.

Review Date: 18 Nov 2020 | Agilent Technologies

Great results.


Average Rating 4.3

Application Area:

Assay development

Great instrument, perfect for small and large laboratories.

Review Date: 18 May 2020 | Agilent Technologies



Average Rating 5.0

Application Area:

Drug discovery, cell study

Reliable instrument with ease of utility. Shows detailed results and provides a greater understanding of cell response for agents.

Review Date: 14 May 2020 | Agilent Technologies

Great imaging capabilities.


Average Rating 5.0

Application Area:

tRNA/DNA quantification

Great imaging capabilities. Very exciting.

Review Date: 28 Jan 2020 | Agilent Technologies

It is one of the best and most useful instrument I have ever used.


Average Rating 5.0

Application Area:


It is one of the best and most useful instrument I have ever used. This device has very wide application and is user friendly.

Review Date: 11 Jan 2019 | Agilent Technologies

The BioTek Agilent Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradeable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows.

The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. Gen5 software provides complete control over all imaging and data capture, plus powerful image and data analysis.

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