High-throughput fluorescent colony formation assay

The colony formation assay evaluates the proliferative capacity of a single cell. Conventional analysis of this assay involves scoring and quantifying colonies in each well of a multi-well format manually by eye, limiting its throughput capabilities.

In this application note, BioTek Instruments presents an automated high throughput method for conducting a colony formation assay in a 96-well microplate using fluorescence microscopy with the Cytation™ 5 wide field of view. This fluorescence-based method is a powerful application of a classic assay, with a streamlined analytical process.