Cloning and Expression Vectors / Systems

Synthetic single guide RNAs for efficient gene knockout & unparalleled specificity Read more...

Synthetic guide RNAs for efficient gene knockout & unparalleled specificity Read more...

Single guide RNA expressing vectors for effective and accurate gene knockout Read more...

Combine sgRNA and Cas9 nuclease expression into a single vector for simplified delivery Read more...

Our CRISPR Design Tool enables an intuitive one-stop location for guide RNA design and ordering. Utilize the design tool to order guide RNAs for targeted gene knockout or location-specific HDR-mediated genome editing. Read more...

Optimize your CRISPR-Cas9 experiments with our specific controls and primers Read more...

A direct and efficient set of tools to study a gene’s function by overexpression in its native context Read more...

For transient gene repression with CRISPR-level specificity Read more...

Horizon's CRISPRi guide RNA in an easy to deliver lentiviral format Read more...

Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in mRNA format Read more...

Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in lentiviral particle format Read more...

A direct and efficient set of tools to study a gene’s function by transcriptional interference in its native context Read more...

Sigma-Aldrich® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including mic... Read more...

With the largest portfolio of Cas9 proteins available, Merck make it easy to find the perfect nuclease for your gene editing experiment.  Read more...

Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequenc... Read more...

The pDual and pDual GC expression vectors are designed for high-level expression of heterologous genes in both eukaryotic and prokaryotic systems. High-level protein expression in bacterial or mammalian systems Saves time by eliminat... Read more...

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-bindin... Read more...

The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, ... Read more...

The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E. coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP puri... Read more...

The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV p... Read more...

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor rec... Read more...

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression. Read more...

Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available ... Read more...

Method completed in less than one day versus 2 days with restriction enzymes Read more...

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in v... Read more...