Application Note: Tumor Cell Killing by T Cells: Quantifying the Impact of a CD19-BiTE Using RealTime Cell Analysis, Flow Cytometry, and Multiplex Immunoassay
28 November 2018

In this application note a combination of impedance-based technology and flow cytometry was used to evaluate both the target and effector cells in a T-cell mediated B cell killing assay. Impedance based Real Time Cell Analysis (RTCA) technology with the xCELLigence® system provided a continuous readout of target cell viability while flow cytometry allowed for simultaneous measurement of specific responses, such as target cell viability and effector cell function.