Analysis of oligonucleotides using ion-pairing alternatives on the Agilent Pro iQ Plus

Oligonucleotides are an emerging therapeutic modality that target and modulate gene expression through the silencing or degradation of mRNA. Ion-pair reversed-phase LC/MS is commonly used to confirm the molecular weights of target oligo sequences to ensure proper synthesis , however, it often requires dedicated instrumentation and toxic and costly perfluorinated alcohols.

Explore an alternative reversed-phase approach that can improve confidence in deconvoluted data. It uses ammonium bicarbonate instead of ion pairing and can provide medium-to high-throughput analysis.