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Whole IgG antibodies are isolated as intact molecules from antisera by affinity chromatography. Read more...
F(ab')2 fragments of antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact some of the hinge region. Used for specific applications, such as to avoid binding of ... Read more...
Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. Block endogenous immunoglobulins on cells, tissues , or other surfaces. Block expo... Read more...
Normal serum can be used as a blocking agent to reduce background. Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze-dried. Normal serum dilut... Read more...
Using biotinylated secondary antibodies along with conjugated streptavidin is a robust means of signal amplification. Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated affinity-purified secondary antibodi... Read more...
Bovine serum albumin (BSA) is used extensively as a carrier protein to dilute antibodies and as a general protein blocking agent in immunoassays and immunodetection protocols. Our IgG-free BSA, which is also protease-free, should a... Read more...
ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals. Read more...
Antisera to Enzymes, Serum Proteins, & Whole Serums. Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide, and fr... Read more...
Solid-Phase Immunoadsorbent Gels. Highly purified IgG from the serum of non-immunized animals is coupled to cyanogen bromide-activated 4% agarose gels for use in preparing affinity-purified antibodies or removing cross-reactive ant... Read more...
Peroxidase-Anti-Peroxidase (PAP) Soluble Immune Complexes. Whole antisera against IgG (H+L) are recommended for bridging PAP to primary antibodies. Antisera against IgG (H+L) or against the F(ab') 2 fragments of IgG will also b... Read more...