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IRDye and VRDye secondary antibodies are highly cross-adsorbed, making them suitable for multi-color detection. IRDye secondary antibodies have been optimized for use with the Odyssey® family of imaging systems and can be used on instrumen... Read more...
When performing two-color detection, loading controls and normalization are essential for reliable, precise comparison of protein levels. LI-COR offers primary antibodies for normalization that can be used for two-color normalization when ... Read more...
IRDye 800CW secondary antibodies are the antibodies of choice for a wide variety of applications in the 800 channel of Odyssey ® Imaging Systems. Read more...
The ChIPAb+ CREB, antibody/primer set includes the CREB, antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 64 bp region flanking the CRE of the human cFos gene. The CREB and negative control cult... Read more...
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Sp1 antib... Read more...
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 anti... Read more...
The ChIPAb+ HDAC1 set includes the HDAC1 antibody, the negative control antibody supernatant (mouse IgG), and qPCR primers flanking an Sp1 binding site in human p21 (WAF1/CIP1/CDKN1A) promoter, amplifying a 105 base pair PCR product. The H... Read more...
The ChIPAb+ Trimethyl-Histone H3 (Lys4) set includes the anti-trimethyl-histone H3 (Lys4) antibody, a negative control antibody (normal rabbit serum), and qPCR primers which amplify a 166 base pair region within the promoter of the human G... Read more...
The ChIPAb+ Acetyl-Histone H3 set contains purified rabbit polyclonal antibody and the normal rabbit IgG antibody, which can be used to demonstrate that the acetyl-histone H3 antibody is capable of precipitating acetyl-histone H3 associate... Read more...
The ChIPAb+ RNA Pol II set includes the RNA Pol II antibody, the negative control antibody (mouse IgG), and qPCR primers flanking the human GAPDH promoter, yielding a 166 bp product. The RNA Pol II and negative control antibodies are suppl... Read more...
Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Trimethyl-Histone H3 (Lys27) set includes the Anti-Trimethyl-Histone H3 (Lys27) antibody, a negative control antibody (purified rabb... Read more...
The ChIPAb+ Acetyl-Histone H4 set includes purified rabbit polyclonal antiserum and the normal rabbit IgG antiserum, which can be used to demonstrate that the acetyl-histone H4 antibody is capable of precipitating acetyl-histone H4 associa... Read more...
The ChIPAb+ Dimethyl-Histone H3 (Lys9) set includes the anti-dimethyl-histone H3 (Lys9) antibody, a negative control antibody (normal rabbit serum), and qPCR primers which amplify a 110 bp region within the promoter of the human β-globin g... Read more...
The ChIPAb+ Ubiquityl-Histone H2B, (clone 56) set includes the Ubiquityl-Histone H2B, (clone 56) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 213 bp region within the coding region of the hum... Read more...
The ChIPAb+ Acetyl-Histone H3 (Lys9) set includes the anti-acetyl-histone H3 (Lys9) antibody, a negative control antibody (purified Rabbit IgG), and qPCR primers flanking an Sp1 binding site in the human p21 (WAF1/CIP1/CDKN1A) promoter, am... Read more...
The ChIPAb+ EZH2, clone AC22 antibody/primer set includes the EZH2, clone AC22 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 135 bp region within the promoter of the human MYT-1 gene. The EZH2... Read more...
The ChIPAb+ Dimethyl-Histone H3 (Lys4) set includes the Anti-dimethyl-Histone H3 (Lys4) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 213 bp region within the coding region of the human GAPDH ... Read more...
TMB, (3,3',5,5'-tetramethybenzidine) has been shown to be a safe-sensitive substrate for the assay of HRP. Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free ra... Read more...
The Luminata ELISA HRP chemiluminescent substrates provide three major advantages: High sensitivity for detecting as little as 0.1 pg of protein Broad dynamic detection range (up to 2,500-fold), enabling a wide range of quantitation. Flexi... Read more...
Penta·His Antibody, BSA-free . For sensitive detection of His-tagged proteins. Kit contents: 100 μg mouse anti-(H)5 (lyophilized, BSA-free, for 1000ml working solution). Benefits: Detection of C-terminal, N-terminal, and internal His tags... Read more...
Penta·His HRP Conjugate Kit. For sensitive, direct detection of His-tagged proteins. Kit contents: 125 μl PentaHis HRP Conjugate, 5 g Blocking Reagent, 50 ml Blocking Reagent Buffer (10x concentrate). Benefits: High-sensitivity and high-s... Read more...
Penta·His Biotin Conjugate. For sensitive detection of His-tagged proteins. Kit contents: 125 μl PentaHis Biotin Conjugate, 200 μg/ml. Benefits: Highly specific and sensitive detection of His-tagged proteins. Flexible detection using any ... Read more...
Tetra·His Antibody, BSA-free . For sensitive detection of His-tagged proteins with HHHH epitopes. Kit contents: 100 g mouse anti-(H)4 (lyophilized, BSA-free, for 1000ml working solution). Benefits: Detection of C-terminal, N-terminal, and... Read more...
Penta·His Alexa Fluor 647 Conjugate. For sensitive, direct immunofluorescent detection of His-tagged proteins, with an emission peak at 647 nm. Kit contents: 125 μl PentaHis Alexa Fluor 647 Conjugate, 200 μg/ml. Benefits: Highly specific ... Read more...
Penta·His Alexa Fluor 488 Conjugate. For sensitive, direct immunofluorescent detection of His-tagged proteins, with an emission peak at 488 nm. Kit contents: 125 μl PentaHis Alexa Fluor 488 Conjugate, 200 μg/ml. Benefits: Highly specific ... Read more...