Longitudinal Characterization of Human Stem Cell-Derived Neurons Using Calcium Imaging
29 Nov 2018

The ability to measure neuronal activity and network activity in a longitudinal manner is paramount to the study of neurological disease. Due to the synaptic plasticity of neuronal cell cultures, identifying dynamic changes between normal and mutated models is critical for understanding changes in network connectivity.

In this webinar, Louis Dang, Clinical Lecturer at the University of Michigan, will:

•Present research findings on evaluating differences in activity between normal and diseased states, using mutant and control human induced pluripotent stem cells (iPSCs) to generate neuronal cultures by forcing expression of Neurogenin-2.
• Describe how the IncuCyte® S3 live-cell analysis system made it possible to measure neuronal activity over the course of several weeks, allowing functional analysis of differences in activity and connectivity.
•Detail research findings that show iPSC-derived neurons had increased activity over time and formed synchronous neuronal networks around three weeks of differentiation.
•Suggest a reproducible, relevant phenotypic readout for long-term network changes in this epileptic cell model.

Sartorius Group