Generating high-quality mRNA-LNP complexes for in vivo delivery: Strategies and methods

Thursday, May 29, 2025 - 9:30 IST | 12:00 SGT | 13:00 KST, JST | 14:00 AEST

The production of high-quality mRNA for therapeutic applications presents several challenges, including yield limitation, low capping efficiency, stability concerns, and immunogenicity issues. In fact, crude mRNA preparations can trigger transient inflammatory responses, complicating their use in clinical settings.

In this expert webinar, we will discuss the latest advancements in mRNA synthesis, purification, and delivery technologies that are helping to overcome these barriers. This session will provide a practical walkthrough of using the mMESSAGE mMACHINE T7 mRNA Kit with CleanCap Reagent AG to achieve efficient co-transcriptional capping and generate high-yield, high-purity mRNA with minimal immunogenic contaminants.

You’ll also discover how Vivofectamine delivery solutions are utilized to encapsulate mRNA into lipid nanoparticles (LNPs) for stable and targeted in vivo delivery. We’ll highlight methods to assess the performance, expression, and immunogenic profile of these mRNA-LNP complexes through in vitro and in vivo assays, including luciferase reporter expression in mouse models.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Webinar details

• Cost: Free to attend
• Location: Online
• Duration: 60 minutes

Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.