Considerations for Selecting the Optimal Stationary Phases for Proteomic Trap-and-Elute NanoLC-MS
24 Jan 2018
The trap-and-elute method for nanoflow LC-MS is a perfect solution to overcome the limitations associated with the small volume of nanocolumns. It allows a comparably large volume of sample to be injected without the risk of sacrificing peak shape or clogging the column.
An optimized trapping condition further increases the method robustness and extends the column lifetime by preventing impurities in samples, such as salts and strongly retained compounds, from entering the nanocolumn. Also, the ability to use different trap and nanocolumn stationary phases provides great flexibility in developing the analytical method for proteomic peptide separations.
Join us to hear Moon Chul Jung, Ph.D., Waters Corporation, discuss:
- Common trap-and-elute system configurations and benefits
- Factors influencing the efficiency of trap-and-elute nanoLC methods
- Understanding and utilizing retentivity of stationary phases