Higher‑quality mRNA starts with pH‑driven dsRNA removal
Wednesday, March 18 at 16:00 GMT | 17:00 CET | 12:00 EDT | 09:00 PDT
The rapid expansion of mRNA‑based therapeutics has intensified the need for highly reliable impurity‑control strategies. Among these impurities, double‑stranded RNA (dsRNA) poses a significant challenge, as even small amounts can trigger unwanted immune responses and compromise product efficacy.
In this SelectScience® webinar, attendees will explore how pH‑based denaturation and Oligo dT purification enable rapid dsRNA disruption, high mRNA recovery, and improved potency. Join Dr. Rok Sekirnik, head of process development of mRNA/pDNA at Sartorius BIA Separations and Evelin Nett, a PhD researcher specializing in RNA‑based technologies, as they share practical guidance on implementing this methodology in modern mRNA production to enhance mRNA integrity for personalized therapies and vaccines.
Key learning objectives:
- Learn how pH denaturation and Oligo dT purification enable selective dsRNA removal while maintaining high mRNA recovery and integrity.
- Understand how optimized dsRNA‑removal workflows can boost downstream performance, including 5-6x improved cell‑based potency.
- Gain insight how to apply this method using HPLC, FPLC, or centrifugation.
Who should attend?
- Scientists and developers working across upstream, downstream, analytical, and process development in mRNA and gene therapy workflows
- R&D leaders, CDMOs, and specialists advancing therapeutic development and manufacturing strategies
Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
Webinar details
- Cost: Free to attend
- Location: Online
- Duration: 60 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.
Speakers
Dr. Rok Sekirnik graduated with a degree in chemistry from the University of Oxford, where he also completed his PhD. His team at Sartorius BIA Separations focuses on developing high-yielding, cost-effective purification approaches for pDNA and mRNA production.
Evelin Nett is a molecular biologist specializing in RNA-based technologies. She earned her Bachelor’s degree in Molecular Biology from Johannes Gutenberg University Mainz and her Master’s degree in Molecular Medicine from Johann Wolfgang Goethe University Frankfurt. She is currently pursuing her PhD at TRON Translational Oncology at the University Medical Center Mainz. Her research focuses on trans-amplifying RNA, with the goal of reducing intrinsic immunogenicity and improving performance for therapeutic and biomedical applications.
Moderator
