Higher‑quality mRNA starts with pH‑driven dsRNA removal

Wednesday, March 18 at 16:00 GMT | 17:00 CET | 12:00 EDT | 09:00 PDT

The rapid expansion of mRNA‑based therapeutics has intensified the need for highly reliable impurity‑control strategies. Among these impurities, double‑stranded RNA (dsRNA) poses a significant challenge, as even small amounts can trigger unwanted immune responses and compromise product efficacy.

In this SelectScience^® webinar, attendees will explore how pH‑based denaturation and Oligo dT purification enable rapid dsRNA disruption, high mRNA recovery, and improved potency. Join Dr. Rok Sekirnik, head of process development of mRNA/pDNA at Sartorius BIA Separations and Evelin Nett, a PhD researcher specializing in RNA‑based technologies, as they share practical guidance on implementing this methodology in modern mRNA production to enhance mRNA integrity for personalized therapies and vaccines.

Key learning objectives:

Learn how pH denaturation and Oligo dT purification enable selective dsRNA removal while maintaining high mRNA recovery and integrity.
Understand how optimized dsRNA‑removal workflows can boost downstream performance, including 5-6x improved cell‑based potency.
Gain insight how to apply this method using HPLC, FPLC, or centrifugation.

Who should attend?

Scientists and developers working across upstream, downstream, analytical, and process development in mRNA and gene therapy workflows
R&D leaders, CDMOs, and specialists advancing therapeutic development and manufacturing strategies

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Webinar details

Cost: Free to attend
Location: Online
Duration: 60 minutes

Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.