Thyroid Hormones in Serum and Plasma Using SPE Extraction and UHPLC-MS/MS Analysis
21 Apr 2016Thyroid hormones play an important role in many biological process such as growth, development and metabolism. Concentrations of circulating thyroid hormones are routinely used to assess thyroid function and diagnose thyroid disease, for correct diagnosis it is essential that the methods for quantifying the thyroid hormones are sensitive and accurate. In this application note a method development is described to measure the free and totally thyroid hormones using a mixed-mode SPE extraction procedure and UHPLCMS/MS analysis.
Related Products
Request Quote for All Products
SELECTRA® HPLC Columns
UCT, Inc.High purity, Type B, spherical silica support. 100A pore size. 1.8um, 3um, & 5um partical sizes. Variety of stationary phases.
ChloroFiltr® for QuEChERS dSPE Clean-Up
UCT, Inc.Chlorophyll in QuEChERS extracts must be removed to reduce potential instrument downtime. This is removed from extracts using graphitized carbon black (GCB) Unfortunately, GCB also removes planar analytes reducing analytical accuracy. ChloroFiltr®, used in place of GCB in dSPE tubes, removes unwanted chlorophyll without removing pesticides or environmental analytes. No change in procedure are required.