SELECTRA® HPLC Columns

This application note outlines a solid-phase extraction (SPE) and LC-MS/MS method for the analysis of 19 synthetic cannabinoids in urine. These specific compounds were selected based on positivity rates from several key testing labs in the area. The use of UCT’s Styre Screen® HLD highly crosslinked polymeric SPE sorbent ensures efficient extraction of the synthetic cannabinoids while removing undesired matrix components and yielding clean extracts

This application note outlines a simple and robust method for the detection and quantitation of 16 cannabinoids in hemp and cannabis samples using an isocratic HPLC method coupled with UV detection. Baseline separation of all 16 cannabinoids, including that of the critical pair Δ9-THC and Δ8-THC, was successfully achieved using a Selectra® C18 column. Examples of hemp flower and oil samples analyzed using the analytical method are also presented. With the widespread legalization of hemp and cannabis, this simple method will be beneficial for any research facility wanting to implement regulatory testing without fighting the wide variation of the matrices and interfering compounds (i.e. terpenes, pigments, etc.) in the diverse products that exist in the marketplace.

This application note outlines a method for the low level determination of aflatoxins in milk using a polymeric solid-phase extraction (SPE) cartridge. Analysis is performed by LC-MS/MS using a Selectra® DA HPLC column. The method was optimized to allow the detection of aflatoxins at the low regulatory concentrations required. Recovery studies were carried out by spiking whole milk at two concentration levels (0.025 and 0.5 µg/kg). Matrix-matched calibration curves, ranging from 0.01-2 µg/kg, were used for quantitation. The mean recovery was found to be in the range of 84 to 100%, and repeatability was ≤7%.

This poster presents chromatograms of EPA method 538 compounds run on a Selectra Aqueous C18 Column.

This application note outlines a direct aqueous injection-liquid chromatography/tandem mass spectrometry method for determination of 11 selected organic contaminants in drinking water. An Aqueous C18 HPLC column was utilized for analyte retention and separation, and calibration curves were constructed using calibration standards prepared in reagent water with preservative reagents for analyte equilibration.

The United States has seen an increased usage and abuse of fentanyl and fentanyl analogs over the last decade, increasing the list of derivatives that forensic toxicologists need the ability to identify. Meanwhile, typically employed immunoassays have proven to be inaccurate for the initial detection of these novel, synthetic drugs.

In this application note, a universal extraction approach for both current compounds and emerging analytes is illustrated, involving a three-step solid phase extraction procedure for the identification of fentanyl and its major urinary metabolite norfentanyl along with eleven additional synthetic opioid compounds.

This application note outlines an all-in-one QuEChERS method for the simultaneous analysis of cannabis for 47 pesticides, 5 mycotoxins and 3 cannabinoids. Sample purification is carried out using UCT’s new cleanup product SpinFiltr™.

Patulin is a naturally occurring mycotoxin that is produced by several species of fungi, such as Aspergillus, Penicillium and Byssochlamys. It typically grows on fruit, including apples, pears, peaches and grapes, but has also been reported in vegetables and cereal grains. Patulin has been implicated as a possible carcinogen and teratogen, although an official designation has not yet been made. The main risk arises when unsound fruit is used for the production of juices and other processed food products. This application note outlines a QuEChERS procedure for the detection of patulin in processed food products.

Benzodiazepines are prescribed for the treatment of anxiety, insomnia, muscle spasms, alcohol withdrawal and seizure prevention. They are also recurrently utilized as illegal recreational drugs and, similar to other commonly abused compounds, “ legal” alternatives have been developed in an attempt to bypass the controlled substances act. This application note describes a method for the simultaneous detection of prescription and synthetic Benzodiazepine in urine using UCT’s Clean Screen® XCEL I extraction columns in conjunction with the Selectra®DA HPLC column.

This application note describes a direct aqueous injection and liquid chromatography tandem mass spectrometry (DAI-LC/MS/MS) method for the determination of cylindrospermopsin (hepatotoxin) and anatoxin-a (neurotoxin) in drinking water under EPA Method 545. These two target analytes are also included in the UCMR4 screening compound list that will be monitored by public drinking water systems soon. Cyanotoxins are toxins that are produced by some species of photosynthetic cyanobacteria (also known as blue-green algae) blooming under certain conditions, such as stagnant or slow moving warm water with high-level nutrients like phosphates and nitrogen.

In this video, Abderrahim Abdelkaoui, application chemist at UCT, discusses analytical solutions for the measurement of per- and polyfluoroalkyl substances (PFAS) in drinking water. Abdelkaoui discusses the lifecycle and impact of PFAS in the environment and optimized methods for detecting PFAS contamination.

Discover the new methods and innovations driving cannabis research forward - plus, a firsthand account of independent cannabis testing in 2020

In this SelectScience special feature, discover the latest methods and technologies for accurate and reliable pesticide and contaminants analysis

We look back at the biggest stories and the best content from our analytical chemistry communities

From field to food discover the technology scientists are using to monitor food and environmental samples in this special feature