Peptide Purification Method Development: Purification of Bivalirudin Using HPLC Columns from Phenomenex
6 Nov 2014The Active Pharmaceutical Ingredient (API), Bivalirudin is a 20 amino acid peptide containing one basic and 5 amino acid residues. It is a direct thrombin inhibitor indicated for use as an anticoagulant in patients. This application note presents the purification method development and scale up experiments performed on a crude sample of Bivalirudin prepared by solid phase synthesis.
Related Products
Request Quote for All Products
Gemini pH Flexible LC Columns
Phenomenex IncSetting the Standard for pH Method DevelopmentRugged reversed phase HPLC columns that offer extended lifetime under extreme pH conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low pH conditions (pH 1-12) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX™ technology Ideal for analytical and preparative separations of basic and acidic compounds Who needs Phenomenex's Gemini HPLC columns? Method Developers: Extreme pH method flexibility Reproducible performance Purification Chemists: Purify bases at high pH Increased loading Increased retention Increased resolution Lab Managers: Extended column lifetime Consistent results.
Synergi Full Range Selectivity LC Columns
Phenomenex IncFull Range SelectivityFour unique phases developed to provide a different selectivity for successful separations of the most complex mixtures and challenging analytes under reversed phase conditions. Whether your compounds are hydrphobic or polar, Synergi columns provide you with a full range of selectivity, ensuring separation of the most challenging and complex mixtures.