Optimization of Caco-2 cell growth and differentiation for drug transport assay studies using Millicell 96-well cell culture plates

This application note demonstrates how to optimize Caco-2 cell culture conditions for reliable drug transport and permeability studies using the Millicell 96-well plates. It outlines protocols for cell growth, seeding density optimization, and monolayer integrity assessment, alongside methods for measuring drug absorption rates. The workflow supports high-throughput screening of candidate compounds and provides reproducible in vitro data to inform drug discovery and ADME evaluation.