ResourceDrug Discovery & Development
Analysis of oligonucleotides using an ion-pairing-free reversed-phase method with TOF LC/MS
29 Apr 2025
The most common method for the analysis of synthetic oligonucleotides is ion-pairing reversed-phase (IPRP) however, it is costly, environmentally harmful, and requires dedicated instrumentation. Explore an alternative method that uses time-of-flight liquid chromatography/mass spectrometry (TOF LC/MS) and a reversed-phase (RP) chromatographic method which enables accurate and repeatable analysis of diverse oligonucleotides without the use of ion-pairing reagents.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.

