Analysis of oligonucleotides using an ion-pairing-free reversed-phase method with TOF LC/MS

29 Apr 2025

The most common method for the analysis of synthetic oligonucleotides is ion-pairing reversed-phase (IPRP) however, it is costly, environmentally harmful, and requires dedicated instrumentation. Explore an alternative method that uses time-of-flight liquid chromatography/mass spectrometry (TOF LC/MS) and a reversed-phase (RP) chromatographic method which enables accurate and repeatable analysis of diverse oligonucleotides without the use of ion-pairing reagents.

Poroshell 120

Agilent Technologies

Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC analyses, and can accelerate your older, traditional 5 µm method for 4-5x reduction in analysis time. With a family of ten chemistries, including the recently introduced PFP, and alignment with the ZORBAX family chemistries, method development and transfer is flexible and easy.Features: High efficiency and high resolution, with up to 50% less backpressure than sub-2 μm columns 2 μm frit, for rugged performance with dirty samples Compatible with 400 bar and 600 bar LCs, as well as UHPLC instruments An expanding family of bonded phases to align with the ZORBAX Family, for reliable scalability Excellent selectivity and peak shapes Designed for exceptional reproducibility Explore Poroshell 120 solutions focused applications, from food to pharma

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.
Analysis of oligonucleotides using an ion-pairing-free reversed-phase method with TOF LC/MS