Miniaturized thermal shift assay of β-lactoglobulin using PULSEspencer R and qTOWER iris 384

This application note demonstrates a miniaturized thermal shift assay (TSA) workflow for assessing protein stability using β‑lactoglobulin as a model system. By combining the PULSEspencer R for precise low‑volume dispensing with the qTOWER iris 384 for UV fluorescence detection, the study shows that reliable and reproducible TSAs can be performed at reduced reaction volumes.

Discover how this approach enables high‑throughput screening while minimizing sample and reagent consumption, without compromising data quality. The results confirm consistent melting temperature measurements across conditions, and highlight the suitability of this workflow for protein analytics and pharmaceutical development applications.