Reverse Transcription PCR (RT-PCR) / cDNA Synthesis Kits Products & Reviews

100 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water

25 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water

Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages

UltraScript® Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.

SensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first strand cDNA synthesis for use in real-time PCR studies (fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, reg…

Real Time RT-PCR: One-Step PrimeScript RT-PCR Kit (Perfect Real Time)

For Greater Specificity, Sensitivity & Yields in cDNA Synthesis and qPCR in a single tube

The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates

igScript™ one step RT-qPCR kit combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity, and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions. igScript™ Reverse Transcriptase is a recombinant MMLV r…

Even with challenging RNA samples, you can get good results quickly and easily. The Invitrogen SuperScript IV One-Step RT-PCR System combines high-processivity Invitrogen SuperScript IV Reverse Transcriptase (RT) and high-fidelity Invitrogen Platinum SuperFi DNA Polymerase to provide super one-step RT-PCR performance.

The High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant transcripts • Liquid format at –10 and –20°C eliminates freeze/thaw cycles and maximizes chem…

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…

Increased amplification – Up to 5,000-fold Greater sensitivity due to increased yields of full-length cDNA GeneChip® quantities of aRNA from only 100 ng input RNA Efficient labeling produces high percent present calls and low 3'/5' ratios Expression profiling using gene arrays to simultaneously measure the expression levels of thousands of genes in a single experiment has become the most widely used method for assessing…

The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enable…

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. Features: High yields of capped RNA—up to 39 µg of RNA per reaction  Extra convenience—cap analog-nucleotide mix provided  Simplified rea…

The Advantage® RT-for-PCR Kit allows first-strand cDNA synthesis from either total or poly A+ RNA. With this kit, you can obtain sufficient quantities of cDNA for PCR from nanogram quantities of any RNA. The Advantage® RT-for- PCR Kit offers several advantages: Longer oligo(dT) primers provide more effective cDNA priming. Recombinant-derived RNase inhibitor minimizes RNA degradation. Each kit contains everything you ne…

Exiqon miRCURY LNA™ First-strand cDNA Kit First-strand cDNA synthesis reagents for high performance microRNA real-time PCR using the miRCURY LNA™ microRNA PCR system. Features of the Exiqon miRCURY LNA™ First-strand cDNA Kit: • Optimized for fast, easy and reliable first-strand cDNA synthesis using our miRCURY LNA™ microRNA PCR system • Includes a thermostable and highly reliable reverse transcriptase The miRCURY LNA™ Fir…

25 x 20 µl reactions, includes 100 µl iScript Advanced 5x Reaction Mix, 25 µl iScript Advanced Reverse Transcriptase

25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 200 µl No-RT control supermix; 12.5 µl DNase; 150 µl DNase buffer

100 x 20 µl reactions, 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 400 µl No-RT control supermix; 50 µl DNase; 150 µl DNase buffer

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

AffinityScript Multiple Temperature Reverse Transcriptase (RT) produces high cDNA yields over the broadest temperature range with high affinity to primer/template complexes. It delivers high cDNA yield when amplifying low RNA input amounts and resolving RNA secondary structure using temperatures up to 55°C.

Easy-A high-fidelity cloning enzymes combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.