Next Generation Sequencing Products & Reviews

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Next-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Twist RNA Exome

Twist Bioscience

The Twist RNA Exome offers a comprehensive solution for capturing human protein-coding regions, enabling efficient transcriptome analysis, isoform study, and fusion detection with high sensitivity and specificity.

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MRD Rapid 500 Panel, 12 Tests

Twist Bioscience

The Twist Bioscience MRD Rapid 500 Panel is a scalable target enrichment solution for monitoring minimal residual disease that leverages Twist’s silicon based DNA synthesis platform to design and manufacture capture panels for personalized medicine. 

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Twist UMI Adapter System

Twist Bioscience

The Twist UMI Adapter System enhances the detection of low-frequency somatic variants in cell-free DNA (cfDNA) by incorporating unique molecular identifiers (UMIs) during library preparation, increasing sensitivity and reducing PCR errors.

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Single cell sequencing

Novogene

Single cell RNA sequencing uncovers the complexity of cellular diversity to employ the study of unusual cell types, cell-lineage associations, and samples of heterogeneous nature with 10x Genomics technology.

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Long-read sequencing

Novogene

Long Read Sequencing (LRS) service employs cutting-edge technologies such as Oxford Nanopore and PacBio platforms for decoding complex genomes, completing transcriptomes, exploring metagenomes, and investigating structural variations with unmatched precision.  

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Shotgun metagenomics sequencing

Novogene

Shotgun metagenomic sequencing is designated to sequence the total genomic DNA from environmental samples without the prior isolation and cultivation of individual species. The services are applied for studying the rich genetic repertoire of microbial communities with taxonomy information, and for studying system evolution, gene function, and metabolic network of environmental microorganisms.

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Epigenome sequencing

Novogene

Novogene provides Whole Genome Bisulfite Sequencing (WGBS), Reduced Representation Bisulfite Sequencing (RRBS), Chromatin Immunoprecipitation Sequencing (ChIP-seq), RNA Immunoprecipitation Sequencing (RIP-seq), and Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq), tailored for both bulk and single-cell samples.

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VeriFi® Hot Start Mix

PCR Biosystems

VeriFi® Hot Start Mix is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

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VeriFi® Hot Start Mix Red

PCR Biosystems

VeriFi® Hot Start Mix Red is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates. The mix includes a convenient red dye for direct gel loading.

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VeriFi® Hot Start Polymerase

PCR Biosystems

VeriFi® Hot Start Polymerase is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

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NGSBIO Library Quant Kit Blue for Illumina

PCR Biosystems

The NGSBIO Library Quant Kit Blue contains all the components required for accurate and sensitive quantification of libraries prepared for Illumina NGS systems. The kit uses an easy-to-see blue qPCR mix to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data.

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VeriFi® Library Amplification Mix

PCR Biosystems

VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

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KAPA RNA HyperPrep Kit

Roche

As our latest generation of RNA library preparation kits, KAPA RNA HyperPrep Kits utilize a novel chemistry and combines enzymatic steps to achieve fewer reaction purifications and yield high-quality RNA-seq libraries in a single day. They also offer flexible workflow options that include the enrichment of desired transcripts by selective mRNA capture or rRNA depletion.

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KAPA HyperExome

Roche

Efficient, and uniform hybridization-based capture for Whole Exome Sequencing Combining more than a decade of probe-design experience with an improved probe-manufacturing process, the new KAPA HyperExome probe pools enable efficient, uniform hybridization-based capture for Whole Exome Sequencing (WES).

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