Next Generation Sequencing Products & Reviews

Streamline the identification of heritable disease-linked alleles, be it through carrier screening or pre- and post-natal testing.

The Twist Alliance Clinical Research Exome helps support the Broad Institute Genomics Platform and was designed using validated data from clinical patient samples.

The Twist Alliance Canine Exome developed in collaboration with the Broad Institute

The Twist Alliance CNTG Exome - 41 MB provides highly uniform coverage of the entire exome as well as full coverage of the mitochondrial genome.

Coverage of rare disease-associated genes 

Coverage of 72 selected cancer-associated genes

The Twist Respiratory Virus Research Panel enables comprehensive detection and sequencing of 29 common respiratory viruses, allowing researchers to identify pathogens and analyze their genomes.

Fast and Easy Library Workflows

Twist's RNA Sequencing Solutions provide comprehensive next-generation sequencing (NGS) workflows, offering both targeted and whole transcriptome approaches to generate uniform, high-quality RNA sequencing libraries from diverse sample types.

The Twist RNA Exome offers a comprehensive solution for capturing human protein-coding regions, enabling efficient transcriptome analysis, isoform study, and fusion detection with high sensitivity and specificity.

The Twist NGS Methylation Detection System provides a robust, end-to-end sample preparation solution for identifying methylated regions in the human genome. The workflow employs a unique enzymatic process from New England Biolabs® that is much less damaging to DNA, alongside Twist‘s Custom Methylation Panel design.

The Twist Bioscience MRD Rapid 500 Panel is a scalable target enrichment solution for monitoring minimal residual disease that leverages Twist’s silicon based DNA synthesis platform to design and manufacture capture panels for personalized medicine. 

The Twist UMI Adapter System enhances the detection of low-frequency somatic variants in cell-free DNA (cfDNA) by incorporating unique molecular identifiers (UMIs) during library preparation, increasing sensitivity and reducing PCR errors.

Clean up and size selection of DNA and RNA for NGS workflows using magnetic beads

Removal of unincorporated terminators from sequencing reactions using magnetic beads

As our latest generation of RNA library preparation kits, KAPA RNA HyperPrep Kits utilize a novel chemistry and combines enzymatic steps to achieve fewer reaction purifications and yield high-quality RNA-seq libraries in a single day. They also offer flexible workflow options that include the enrichment of desired transcripts by selective mRNA capture or rRNA depletion.

Efficient, and uniform hybridization-based capture for Whole Exome Sequencing Combining more than a decade of probe-design experience with an improved probe-manufacturing process, the new KAPA HyperExome probe pools enable efficient, uniform hybridization-based capture for Whole Exome Sequencing (WES).

KAPA HyperPETE (Primer Extension Target Enrichment) is a novel hybridization capture technology designed to employ primer extension reactions to specifically capture and release target library molecules for sequencing.

KAPA EvoPlus V2 Kits is an enhanced enzymatic DNA library preparation solution that features improved fragmentation performance, insensitivity to inhibitors, increased conversion efficiency & fewer sequencing artifacts.

Achieve high-performing NGS library preparation from mechanically fragmented DNA or cell-free DNA (cfDNA) in a streamlined, automation-friendly workflow with KAPA EvoPrep Kit. The KAPA EvoPrep workflow reduces pipetting and reagent preparation steps and is validated with challenging sample inputs such as cfDNA and FFPET DNA.

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high-fidelity polymerases and polymerase blends.

Automated Purification of High-Quality Total RNA with an Enhanced miRNA Enrichment

Double-stranded DNA (dsDNA) library prep for enzymatic fragmentation. Includes library prep and polymerase for amplification. Available in two configurations: The xGen™ DNA Library Prep EZ Kits include xGen™ Stubby Y Adapters for indexing by PCR workflows (indexing primers must be purchased separately). The xGen™ DNA Library EZ UNI Kit requires the purchase of full-length, indexed adapters for PCR-free indexing by ligation wor…

Double-stranded DNA (dsDNA) library prep for enzymatic fragmentation. Includes library prep and polymerase for amplification. Available in two configurations: The xGen™ DNA Library Prep EZ Kits include xGen™ Stubby Y Adapters for indexing by PCR workflows (indexing primers must be purchased separately). The xGen™ DNA Library EZ UNI Kit requires the purchase of full-length, indexed adapters for PCR-free indexing by ligation wor…

Double-stranded DNA (dsDNA) library prep for enzymatic fragmentation. Includes library prep and polymerase for amplification. Available in two configurations: The xGen™ DNA Library Prep EZ Kits include xGen™ Stubby Y Adapters for indexing by PCR workflows (indexing primers must be purchased separately). The xGen™ DNA Library EZ UNI Kit requires the purchase of full-length, indexed adapters for PCR-free indexing by ligation wor…