Juno™
Targeted NGS library preparation, gene expression and genotyping. Increase productivity and efficiency with automated, cost-effective, and easy-to-use workflows for targeted DNA next-generation sequencing (NGS) library preparation, gene expression analysis and genotyping by allele-specific PCR.
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Using the Juno™ Targeted DNA Sequencing Library Preparation System, including Targeted DNA Seq Library Preparation reagents, you can produce dozens to hundreds of sample libraries daily, with each sample enriched for up to 4,800 specific amplicons covering user-defined genes or genomic loci. Optimized for use with Illumina® sequencing systems in combination with Standard BioTools™-supplied sample barcodes, Juno enables accurate sequencing of more samples—faster and more affordably than ever before.
Juno also serves as a universal controller to prepare integrated fluidic circuits (IFCs) for both gene expression and genotyping analysis when used together with the Standard BioTools Biomark™ HD system. While providing significant advantages in sample throughput for the analysis of dozens to hundreds of DNA variants or RNA transcripts per sample, the Juno system also significantly reduces reaction volumes and required hands-on time, resulting in substantial savings over traditional methods.
- Scale - Generate dozens to hundreds of NGS-ready libraries in a single run using Juno and either the 48 sample or 192 sample Library Prep IFC.
- Save - Boost affordability by reducing reagent and sample volumes and labor requirements, for lower overall cost.
- Simplify - Easily design assay panels for targeted NGS, SNP genotyping or gene expression to detect DNA variants, mRNAs or miRNAs—from a few targets to several thousand.
Flyer: Advanta RNA-Seq NGS Library Prep Kit
Designed to drive significant improvement in the RNA-seq workflow, the Advanta™ RNA-Seq NGS Library Prep Kit together with the Juno™ system provides an integrated solution for automated NGS library prep. This flyer from Fluidigm demonstrates the capabilities of the microfluidics-based workflow.
Maximizing workflow efficiencies for robust RNA-Seq library preparation
RNA sequencing (RNA-seq) has become the gold standard for expression profiling methods. However, RNA-seq protocols can be time-consuming and costly with increasing numbers of samples. In this application note, Fluidigm describes the comprehensive performance characteristics of the Advanta RNA-Seq Kit and Juno system generated from internal studies.
Developing solid tumor and hematological panels for a flexible and automated microfluidic workflow that improves efficiency of NGS library preparation for cancer research
In this study, Fluidigm designed two amplicon-based, targeted NGS library preparation (LP) panels for detecting single-nucleotide variants, insertions and deletions, copy number variants, and RNA fusions. This workflow, coupled with relevant panel content and performance, offers a solution for investigators requiring economical NGS testing of FFPE and blood samples.
Detection of human and viral full-length RNA using a nanoscale microfluidic platform
RNA sequencing (RNA-seq) has become the gold standard of expression profiling methods. In this study, Fluidigm have developed an elegant microfluidics-based chemistry and workflow called the Advanta™ RNA-Seq NGS Library Prep Kit (PN 101-9187). Leveraging the Juno™ instrument, this RNA-Seq Kit is designed to support simultaneous processing of up to 48 samples with a one-click script.
Fluidigm Corporation's core technologies
In this video, Fluidigm gives insights into their core technologies, designed to power health insights by delivering comprehensive multi-omic solutions.
Detection of human and viral full-length RNA using a nanoscale microfluidic platform
In this video, Fluidigm Corporation presentes their virtual scientific poster on the detection of human and viral full-length RNA using the Juno™ and the Advanta RNA-Seq NGS Library Prep Kit.
