High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
BetaMax Acid Columns o High surface area area and bonded phase coverage for maximum retention, even in high percentage organic conditionso Chemistries for acidic compounds Product detail: For alternative selectivity to traditional alkyl phases, we recommend you try the polar embedded phase BetaMax columns. The stationary phase contains urea groups embedded within the C12 chain, which extra interactions with samples to…
BETASIL C18 Columnso Broad range of particle sizes and phases for wide method development optionso High surface area and phase coverage for reproducibility and sharp peak shapes Product Detail BETASIL columns are based on 100Å pore size, fully deactivated type B silica to ensure sharp peaks and excellent reproducibility for the analysis of a wide range of compounds. The 100Å pore size gives a higher concentration of statio…
BetaBasic 4 Columnso Excellent peak shapes for basic compoundso Superb stability at both low and high pH Product detail:BetaBasic 4 columns are based on a 150Å pore size, fully deactivated type B pure silica to ensure sharp peaks and excellent reproducibility for the analysis of basic compounds. Due to stable bonding chemistry and excellent surface coverage, BetaBasic 4 columns show excellent peak shape for basic compound…
Hyperbond™ is a cost effective alternative to µBondapak (a trademark of Waters Corporation). It is based on an irregular silica with 10µm particle size.
o 100% porous graphitic carbon with very different selectivity to reversed-phase silica columnso Exceptional retention of very polar compoundso Separation of structurally similar compoundso Stable from pH 1 to 14 at high temperatures in a wide range of solvents Product detail: Hypercarb is a unique material for HPLC manufactured by Thermo Hypersil-Keystone. Hypercarb consists of fully porous spherical carbon particle…
o Separate hydrophobic and ionic compounds in a single runo Select from two mixed mode phases, using either cation or anion exchange combined with C18o Retention of compounds is optimized using mobile phase pH, ionic strength and percent organic Product detail: Hypersil Duet columns are an ideal choice for unknown compounds and metabolism studies, as they can separate hydrophobic and ionic compounds in a single analysis…
The Hypersil SAX is recommended for analysis of small organic molecules and organic acids. Product detail: Hypersil SAX is a strong anion exchange column, which is typically used for analysis of small organic molecules and organic acids. As the columns are based on the first generation classical 120Å pore size silica they are not recommended for analysis of biomolecules. For new methods we recommend trying our latest ion…
Thermo Electron Hot and Cool Pockets™ offer unbeatable value in coulmn temperature control. The pockets are small and light weight yet offer precise temperature control. Hot pockerts heat from 5 to 85 oC with a temperature accuracy of ±2oC over the entir range, and a temperature stability of ±0.1Oc. Hot Pockets™ and Cool Pockets™ are not available within the European Union at the present time. We apologise for any inconvenien…
A new screw cap that has been introduced for HP users, to enable them to use a screw top vial on a robotic arm autosampler.
Achieve faster run times and improved sensitivity with a smaller particle size
ACQUITY UPLC® BEH Phenyl columns utilize a trifunctional C6 alkyl tether between the phenyl ring and the silyl functionality. This ligand, combined with the same proprietary endcapping processes as the ACQUITY UPLC™ BEH C18 and C8 columns, provides ultra-low column bleed, long column lifetimes and excellent peak shape. This unique combination of ligand and endcap on the 1.7 μm BEH particle creates a new dimension in selectivi…
The Waters IdentityE High Definition Proteomics system defines the new standard for rigorous protein identification. The IdentityE High Definition Proteomics System is an UltraPerformance LC® (UPLC)/High-Bandwidth Mass Spectrometry (MS E) system solution suitable for confident protein identification designed to meet stringent requirements as outlined by leading scientific journals.The new Waters IdentityE High Definition Pro…
The ACQUITY UPLC® Fluorescence (FLR) Detector brings unrivaled sensitivity and selectivity to UPLC®. The detector’s innovative flow-cell design, low-noise electronics and support for high-speed data rates are perfectly suited to the efficient separations of the ACQUITY UPLC's advanced fluidics technology. Target applications include PAHs, environmental contaminants, vitamins, aflatoxins and pharmaceuticals. Ultra Performance i…
SymmetryShield™ Columns feature Waters' patented embedded polar group technology that shields the silica's residual silanols from highly basic analytes at improves overall peak shape. Additionally, by placing the embedded polar group close to the silica surface, the activity of the surface silanols is further reduced. This imparts selectivity and retention that is different compared to the Symmetry C18 ligand. Embedded polar…
Polynuclear Aromatic Hydorcarbons (PAHs) are among the most frequently monitored environmental contaminants. Waters PAH columns are optimized for the HPLC analysis of PAHs. These columns achieve baseline resolution and excellent peak symmetry for 16 different target analytes in less than 25 minutes, while employing a simple water/acetonitrile binary gradient method. The resolving power of the PAH columns provides superior pe…
The popular Bondapak® C18 chemistry and Porasil® silica packing material are available in three different particle sizes, 10, 15-20, 37-55 micron, providing easy transfer of chromatography methodology from one particle size to another. You can utilize your existing 10 micron Bondapak or Porasil chromatography as a starting point for scale-up or, if you are developing a new purification protocol, investigate which preparative…
These columns provide accurate and precise molecular weight calculations in under 7 minutes! - Fast, accurate and precise molecular weight determinations - Increase sample throughput - Greatly decrease solvent consumption and disposal
The Envirogel high-efficiency GPC cleanup columns are specifically designed to remove low volatility, high molecular weight interferences, such as lipids and natural resins, from environmental samples as specified in EPA Method 3640A. In the past, the cleanup procedure for environmental samples was performed on low-efficiency GPC columns based on packing particle diameters of 37-75 micron (200 to 400 mesh) Bio-Beads S-X resins…
For applications where the sample is neither volume or concentration limited, Waters offers 1 and 2mm Internal Diameter columns of various lengths for use on traditional HPLC MS inlets.As with Waters array of NanoEase™ columns, the same Waters reversed-phase packings are also available in these two column internal diameter offerings. Chemistry choices include: XBridge® Columns, CORTECS® Columns, XSelect Columns, Atlantis® Pola…
MassPREP™ On-Line Desalting Cartridges are an effective tool for the successful desalting of acidic, basic, and large globular proteins. The overall MassPREP™ desalting strategy provides an easy, fast (< 3 minutes), and reproducible approach for the effective desalting of proteins stored in physiological salt buffers prior to high resolution ESI-MS structural analysis.
The polar organic and the polar ionic modes have become increasingly popular largely because of their ideal compatibility with MS detection and preparative LC. Two years of extensive research at Astec in understanding these mechanisms has resulted in new possibilities for extending their effectiveness even further. Important changes in linkage chemistries and the silica and the silica have now led to the new CHIROBIOTIC V2 and…
The DE Midi range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, primarily in Development, who are looking purify samples from 5 grams to 1,000 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…
