Gene Expression and Molecular Cloning Products & Reviews

Gibson Assembly® allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility.

PD-L2/TCR Activator Mammalian Expression Kit

Visium Spatial Gene Expression is a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context in FFPE or fresh-frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research.

Genotyping by sequencing (GBS) is the cost-effective method of choice for genome-wide SNP discovery without prior knowledge of the genome sequence.

The LightCycler® 96 Instrument is a real-time PCR instrument for rapid cycling up to 96 samples. Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping. Precise LightCycler® 96 Instrument thermal homogeneity and cycling speed produce accurate and reproducible results in a very short time. The LightCycler® 96 Instrument’s optical detection system flexibly detects sequenc…

Human iPSC-derived glutamatergic neurons expressing Cas9 for rapid gene knockout generation

PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus

The High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant transcripts • Liquid format at –10 and –20°C eliminates freeze/thaw cycles and maximizes chem…

These bacterial expression vectors carry the gene for either GFP (wild type Aequorea victoria green fluorescent protein) or GFPuv (a GFP variant optimized for maximal fluorescence when excited by UV light [360–400 nm]) and are driven by the lac promoter. These vectors can also be used as a source of the indicated fluorescent protein coding sequences. The fluorescent protein gene can be amplified by PCR or readily excised using…

The Model P-97 micropipette puller is the latest generation of the Flaming/Brown type puller for fabrication of micropipettes, patch pipettes and microinjection needles. While retaining many of the features on the Model P-87, the P-97 offers improvements in mechanical, electronic and software design. The result is better control of the pulling process and a higher degree of reproducibility. Features: Environmental chamber…

INTERFERin™-HTS is a new generation siRNA transfection reagent especially developed for high throughput screening (HTS) applications providing great silencing efficiency, excellent reproducibility and high cell viability with very low amounts of reagent. INTERFERin™-HTS is cost-effective, easy to handle, compatible with serum and antibiotics, and comes with reverse and forward protocols for 96- and 384-well plates. Great s…

100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

Gene Pulser®/Micropulser™ electroporation cuvettes are high-quality electroporation cuvettes which enable reproducible and consistent gene delivery to valuable transfection samples.

The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunoprecipitation (ChIP) pull-down coupled with powerful DNA microarray location analysis. Beyond embryonic and adult development gene regulation, Agilent™s ChIP-on-chip model systems effectively elucidates the vital biological processes.  Probe number depends on species  4x44k and…

The Agilent Rabbit Microarray delivers accurate, consistent and efficient gene expression profiling of rabbit samples. The transcriptome-wide microarrays enable scientists to study gene expression profiling of Oryctolagus cuniculus samples at the global level and enhance the understanding of gene function.

The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. 

FuGENE® HD Transfection Reagent is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in che…

The Dual-Luciferase® Reporter (DLR™) Assay System(a–f) provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at l…

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize s…

The CosMx Spatial Molecular Imager is an FFPE-compatible, single-cell imaging solution that enables researchers to investigate intact tissues through the power of spatial multiomics.

The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format. What benefits are important for your work? Efficient transfection in single cuvette format? Up to 90% efficiency with plasmid DNA…

In combination with the included Enhancer Solution, the reagent efficiently delivers functionally active proteins, antibodies and peptides into the cytoplasm of cells. Proteins of different sizes and net charge (different pI) can be delivered into various cell types, including primary, adherent and suspension cells (see Fig. 1, 2 and list of cells below). The reagent can be used in the presence or absence of serum. TurboFect P…

The Yeastmaker Yeast Transformation System 2 provides a high-efficiency polyethylene glycol (PEG)/LiAc-based method for preparing and transforming competent yeast cells. The Yeast Transformation System 2 protocol provides a higher and more reliable transformation efficiency than many other commonly used methods as a result of specially formulated "YPD Plus" recovery medium, which results in more surviving transformed colonies…