UHPLC and HPLC > Column Supplies Products & Reviews

Synthetic silica, composed primarily of silicon dioxide, has become indispensable in today’s world in regard to its exceptional chemical stability. Consequently, uncountable commercial applications are now relying on synthetic silica, for instance, it is the most widely used stationary phase for chromatography. For nearly two decades, SiliCycle has been exploring silica’s great possibilities and versatility in order to chall…

The 1.7 µm Ethylene Bridged Hybrid [BEH] particle is one of the key enablers behind UPLC® technology.It is available in three different pore sizes [130Å, 200Å and 300Å] and several bonded phases for reversed-phase and hydrophilic interaction chromatography and is applicable from small molecule to large biopharmaceutical analysis. Due to the intrinsic chemical stability of hybrid particle technology, a wider usable pH range [pH…

Glycan columns for the analysis of released N-labeled glycans using UPLC and with MaxPeak High Performance Surfaces to reduce non-specific adsorption.

Glycan columns for the analysis of released N-labeled glycans using HPLC and with MaxPeak High Performance Surfaces to reduce non-specific adsorption.

Anion exchange, reverse-phase columns that provide charged-based separations and extra resolution for acidic glycans. With MaxPeak High Performance surfaces that reduce non-specific adsorption.

These columns are been purposefully designed for intact glycoprotein profiling, subunit fragment analysis, and glycopeptide mapping using hydrophilic interaction chromatography (HILIC). 

Designed for reverse-phase separation of amino acids derivatized with AccQ•Tag Ultra reagent. Rapid separation with a superior resolution for HPLC/UHPLC. 

Size-based separations of peptides, proteins, and viral vectors

Reversed-phase based separations of peptides

The VanGuard FIT column format utilizes a unique, easily replaceable Fully Integrated Technology (FIT) pre-column cartridge that avoids added dispersion and facilitaes easy method transfer of existing methods run on standard columns.  

Size-based separations of peptides. proteins, and viral vectors

Over 50 years of reproducible performance

The popular Bondapak® C18 chemistry and Porasil® silica packing material are available in three different particle sizes, 10, 15-20, 37-55 micron, providing easy transfer of chromatography methodology from one particle size to another. You can utilize your existing 10 micron Bondapak or Porasil chromatography as a starting point for scale-up or, if you are developing a new purification protocol, investigate which preparative…

The 1260 Infinity II Preparative Column Organizer is a support module for purification workflows. It provides convenient and stable mounting of a manual injector and easily organizes both analytical and preparative columns from 4.6 to 50 mm inner diameter.

Use our bulk packing materials to pack your own columns. Our extensive QC program ensures the high quality and reproducibility of these silicas. You can be confident that you are getting consistent, high-quality product when you source your silica from Restek. Use these materials for easy scale-up to preparative chromatography or for packing your own columns. Ultra Bulk Packing Materials Features: Prepare your own columns…

This new strong anion exchange resin offers an optimised macro-porous structure that ensures an efficient purification of large biomolecules, such as adeno-associated viruses (AAVs) and plasmid DNA (pDNA). With its high binding capacity for such molecules and high separation efficiency even at elevated flow rates, MacroSep IEX Q is a perfect addition to DSP platforms.

BioPro IEX, YMC's strong ion exchange material are based on a hydrophilic polymer which provides low non-specific adsorption and high recoveries. Porous and non-porous polymer beads are available for high loadability and throughput.  

With YMC-Triart Prep, pH stable preparative HPLC stationary phases are available for industrial-scale applications. This hybrid silica-based material can be used in a pH range of 2.0 - 10.0 and allows more efficient cleaning procedures. The innovative YMC-Triart Prep combines mechanical strength and pH stability.

BioPro IEX and BioPro IEX SmartSep resins are specifically designed for biochromatography. They are available as a strong anion exchanger (BioPro IEX Q) and a strong cation exchanger (BioPro IEX S). The resins are based on a hydrophilic polymer matrix, with a particle size of 10 µm, 20 µm, 30 µm and 75 µm.

Highly robust hybrid silica material for the purification of proteins, peptides and oligonucleotides, which combines mechanical strength and pH stability. The larger pore size makes YMC Triart-Prep Bio200 C8 the ideal choice for the purification of mid-size to large biomolecules.

Endrix, the next evolution of Phenomenex Kinetex and Luna Omega LC columns, is engineered to maximize instrument uptime. Its proprietary hardware delivers unmatched consistent and reliable performance on matrix-rich samples, supporting seamless LC-MS/MS workflows without time-consuming clean-up steps.

Disks, Cartridges, well plates and more are available using a unique PTFE matrix membrane for unparalelled SPE

Designed to take advantage of low dispersion, ultra-high pressure UHPLC instruments, Avantor® ACE® Excel UHPLC columns give chromatographers more choices to achieve better results

Offers a wide range of bonded phases for every separation challenge

Evosphere® monodisperse fully porous particle columns lower band broadening by improving the homogeneity of the packing bed of the column, reducing the van-deemter curve, leading to increased efficiency and selectivity.