Life Sciences Products & Reviews

The new Mastercycler® X50 elegantly combines speed, flexibility, and ease of use. With its intuitive touch screen offering precise fingertip control, the MC X50 PCR thermocycler enables easy PCR optimization in advanced molecular biology research and dependable standardization in routine PCR applications. It is also perfectly fitted for NGS.

Eppendorf real-time PCR Tube Strips feature an extremely thin wall for optimal heat transfer combined with high mechanical stability. In combination with their optical properties these are the ideal tube strips for any real-time PCR, especially for use with small reaction volumes. The Masterclear™ Cap Strips offer further advantages for your real-time PCR. The inverted dome of the Masterclear™ Cap Strips prevents scratching or…

Welcome the Eppendorf Eporator®, the solution for efficiently transforming bacteria and yeast! The Eppendorf Eporator is the perfect fit for you and your lab. Ask the specialists – your bacteria and yeast – they will confirm that the product features and performance of the Eppendorf Eporator make it very easy for you to carry out your experiments and obtain excellent results. Electroporation can be used for the transformatio…

Clean Screen® Xcel Well Plates The Clean Screen Xcel products are a line of sample prep phases designed to reduce the number of steps in the extraction, and therefore the amount of time and solvent necessary to complete the sample cleanup. Further advantages include reduced sample size and improved cleanliness and recovery. The Xcel I well plate will extract a wide array of basic drugs including benzodiazepines and opiates.…

Positive Pressure Manifold - A simple, robust way to quickly process up to 48 samples at one time. The Positive Pressure Manifold can accommodate a multitude of column sizes and provides even flow to each position. Positive Pressure Manifold Features: All 48 positions are individually regulated to provide even pressure to each column. There are four banks of 12 positions each. The user can control flow to each of the four…

SELECTRA-SIL® Derivatizing Reagents SELECTRA-SIL® is an extensive line of derivatizing reagents manufactured by UCT at its facility in Bristol, PA. Our reagents are designed to increase molecular weight, lower boiling points, and create a more specific ion for compounds intended for gas chromatography and GC/MS. SELECTRA-SIL® Derivatizing Reagents Benefits: Improved chromatographic resolution - increased volatility - red…

Our 96 Deep Well Plates are made from solvent resistant, low extractable polypropylene. Standard frits are polyethylene with 20μm pores. The 2mL well provides capability for methods that require a larger sample size. The separate wells and greater capacity eliminate well-to-well carry over. We offer disposable plates compatible with Beckman, Caliper, Gilson, Hamilton, PerkinElmer and Tecan liquid handling systems. 96 Deep Wel…

The DPPIV-Glo™ Protease Assay is a homogeneous, luminescent assay that measures dipeptidyl peptidase IV (DPPIV) activity. DPPIV is a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position. The DPPIV-Glo™ Assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. The addition of a…

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents…

The ApoTox-Glo™ Triplex Assay combines three assay chemistries to easily assess viability, cytotoxicity and apoptosis events in the same cell-based assay well. First, viability and cytotoxicity are determined by measuring two differential protease biomarkers simultaneously with the addition of a single nonlytic reagent containing two peptide substrates. The live-cell protease activity is restricted to intact viable cells and i…

The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence of HRP. The ECL Western Blotting Substrate is a highly sensitive non-radioactive,…

PCR Master Mix(a,b) includes Nuclease-Free Water and PCR Master Mix, 2X. PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. The PCR Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2–2kb.

The Griess Reagent System measures nitrite (NO2–), which is one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many mod…

The QuantiFluor™ RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is a very important step used in many biological applications, particularly in molecular biology techniques. These include measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot anal…

The GoTaq®Real-Time PCR Systems are ready-to-use 2X master mixes optimized for quantitative PCR in dye (using BRYT(R) Green Dye) or hydrolysis probe detection format.   The 1-Step and 2-Step RT-qPCR Master Mixes contain GoScript™ Reverse Transcriptase for efficient first-strand cDNA synthesis.     GoTaq Real-Time PCR Systems Features & Benefits: Higher fluorescence, often resulting in earlier Cqs Direct su…

The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use.   The PowerPlex® Fusion System provides all of the materials needed for co-amplification and five-color fluorescent detection of 24 loci (23 STR loci and Amelogenin), including the CODIS core loci and the European Standard Set (ESS) loci. With 24 loci, the sys…

The Wizard® Genomic DNA Purification Kit provides a simple, solution-based method for isolation of DNA from white blood cells, tissue culture cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative bacteria.   DNA purified with this system is suitable for a variety of applications, including amplification, digestion with restriction endonucleases and membrane hybridizations (e.g., Southern and dot/s…

The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimi…

The ROS-Glo™ H2O2 Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions.   This assay allows identification of conditions or test compounds, such as small molecule inhibitors or inducers, that alter ROS levels. The scalable multiwell format couples a stable luminescent si…

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.   Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages,…

Polyclonal secondary antibodies raised in goat, rabbit or donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme.   The Anti-Human IgG (H+L), HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate antibodies bind to both heavy and light chains for all IgG subclasses. As with all antibodies, in certain applications…

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.   The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).   Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…

The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes.   The proprietary column/binding matrix can efficiently capture RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl). Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or fro…

The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests.   Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, wh…