Products & ReviewLife Sciences

Human IL-8 ELISA

RayBio® Human IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, and serum samples.

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RayBiotech Inc.

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Average Rating 4.7

|2Scientists have reviewed this product

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Great results, my number one option for ELISA assays!


Average Rating 5.0

Application Area:

Analysis of human colon cancer cell's lines in response to inflammation

This product is very effective and easy to use. The instructions are easy to follow, the sensitivity is in pg/ml (very low concentrations are detected) and are more affordable than other vendors.

Review Date: 22 Jun 2018 | RayBiotech Inc.

Great results! I will use it again


Average Rating 4.3

Application Area:

Measurement of cytokine secretion in colon cancer cells

This ELISA IL-8 is great! It gave good results and they matched what I expected to see in my colon cancer cells. The tech support was easy to talk to and very helpful. This ELISA is easy to use and the protocol steps are very straight forward. In addition, the price is adequate and better than the competitors. I will definitely buy more of these ELISA kits!

Review Date: 19 Jul 2017 | RayBiotech Inc.

Assay Format

Detection Range

1 pg/ml - 600 pg/ml

Recommended Dilution (serum & plasma)

2 fold


1 pg/ml


This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Species Detected


Compatible Sample Types

Cell Culture Supernatants, Plasma, Serum

Design Principle


Method of Detection




Solid Support

96-well Microplate

Protein Information

Accession Number


Gene ID


Gene Symbols


Protein Name & Synonyms

Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I) Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b) IL-8(5-77) IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF) IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV) IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V) IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]

Target Species


Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Product Overview