xCELLigence RTCA SP by ACEA Biosciences – A part of Agilent

Manufacturer ACEA Biosciences – A part of Agilent  |  Available Worldwide
4.2
/
5.0
  |  12 reviews
Label-free, real-time cell analysis in a 96-well format. The xCELLigence® RTCA SP instrument uses noninvasive electrical impedance monitoring to quantify cell proliferation, morphology change, and attachment quality in a label-free, real-time manner.  The SP (single plate) model differs from our other xCELLigence instruments in that it uses a 96-well electronic microtiter plate (E-Plate® 96).  The instrument is placed in a standard CO2 cell culture incubator and interfaces via a cable with an... Read more


xCELLigence RTCA SP by ACEA Biosciences – A part of Agilent product image
xCELLigence RTCA SP
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Average Rating: 4.2
12 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: INRS-IAF



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
RTCA permitted to test a large number of samples which is time-saving and economical.
Rating: 4.0

  • Application Area: proliferation, cytotoxicity

"The ACEA’s platform technology was used with E-plates to monitor the proliferation of human placental cell line JEG3, HIPEC and BeWo treated with increasing concentrations of SSRIs. This technique is a unique and pioneering approach to test the safety of drugs prescribed during pregnancy on placentation. The xCELLigence system has provided crucial data on the potential cytotoxicity and proliferative effects of antidepressants in real time. This technology is ideal for IC50 and AUC calculation. RTCA-SP and its 96-well E-plates permitted to test a large number of samples in the same experiment, which is time-saving and economical."

Review date: 25 Jul 2018 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Saarland University



  • Ease of use
    4 out of 5
    After sales service
    1 out of 5
    Value for money
    4 out of 5
We are not satisfied with our system any more and not at all with service.
Rating: 3.0

  • Application Area:Analyze the effects of gene silencing and small molecules, respectively, on different cell lines

"In principle, the product is really good. We were satisfied for a couple of years. However, in the course of the last two years we experienced serious problems with the measurements in more and more wells and spent two times almost 10,000.- € to get the problems solved. It did not work out as hoped. By the end of last year, our system was serviced back at ACEA in the US. This took almost 6 months. When we got it back, the problems were still the same."

Review date: 19 Jul 2018 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Erciyes University Faculty of Pharmacy, Division of Pharmaceutical Sciences (Head), Department of Ph



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
In conclusion technical and scientific support should be improved.
Rating: 3.7

  • Application Area:To investigate the effects of a novel natural or synthetic drug candidate on cell survival.

"We achieve high-quality results but our results are sometimes not reproducible. We try to seed the cells in the same passage but the profiles of the curves or the max cell indexes with the same cell do not fit with each other. When the curves ar enot similar each time the effects of our compounds that we are testing also alters and this is one of the most common problems we have with the system. To test our results we try to confirm our results with regular cytotoxicity tests but usually it fails. Because our results sometimes does not fit the common cytotoxicity tests although we perform these tests in 1st, 2nd 3rd,6th,12th and 24th hour trials to mimic "real time" effects. When the results are not reproducible we need to compare our xcelligence results with regular cytotoxicity tests each time which makes us sometimes unable to go further molecular experiments before identifying these different responses with different methods. The plates are expensive therefore sometimes we have to limit our experimental plans that we do not have enough financial support to consume many e-plates. And this limits us to have reproducible results. The system is simple but the software might be improved a little bit more to achieve a customer friendly way of use. We sometimes get difficulty to discuss our results since the effects of each compound we try, have different effects on the profile of the curves but these styles are not identified clearly with the molecular mechanisms. And we sometimes get difficulty in publising our results with xcelligence system becuase the reviewers usually see this system for the first time and ask many questions and sometimes need us only to reflect the cell index results of one end point such as 24th hour after application. So usually we need assistance in discussing our results while writing papers and we need to be supported during our publication process. The impedence sometimes alters because of the morphology of the cells not the number of the cells we use e-view plates to see whether these changes are due to morphology or the number of the cells but the system can be improved making us able to visualise what happens to cells in a real time manner without taking the plate of the system. To achive this an adoptation of a camera system should be very useful and helpful. We are pleased from the customer meetings every year being held by the distrubuter firm in Turkiye and we are pleased from the contributions of Yama Abassi. But he is for sure so busy sometimes to deal with all the customers all over the world therefore the firm should increase the number of the scientific people to discuss our results before publication since only one person cannot reach everybody each time. The webinars are helpfull to cope with this problem but might be more frequent gathering all the responses to the questions that the customers have. The firm should supply a technical support for validation of the system at least once a year. The technical assisstance was satisfactory in the first years but gets poorer in Turkiye. As an overall comment thanks to ACEA for Xcelligence system but we need more scientific and technical asistance."

Review date: 19 Jul 2018 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: AgResearch



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Great results, we use it routinely, even though consumables can be quite expensive.
Rating: 3.7

  • Application Area:Analyse cell growth kinetics

"Excellent instrument for non-invasive label-free real-time monitoring of cell growth kinetics. We use the cell index, a compound parameter (including cell morphology, size and proliferation/death rates) as QC for all of our cell lines. Any changes in culture conditions, passage number etc are picked up by deviations from the standard. The new 96-dishes allow visualization of cells, which is very useful. Pharmacological screens are easy and high-throughput. Sufficient numbers of technical replicates are easily achieved given the plate design. Instrument is very easy to use and data analysis is straightforward. My only concern is that the cable that connects the device through the incubator door can quite easily break."

Review date: 19 Jul 2018 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: CNRS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy to use, original results, fast and funny to use.
Rating: 5.0

  • Application Area:cell proliferation, adipocyte lipogenesis, toxicity of nanoparticles

"Xcelligence give the vision on the experiments. For some time at the begining it was only descriptive but it also allows to investigate the signaling pathways of the regulation studied. I use Xcelligence on a daily basis. I performed a lot of experiments (/ex testing different cuture medium) that I would have never done without it, because Xcelligence is fascinating giving real-time results without labelling."

Review date: 18 Jul 2018 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Acepodia biotech



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great instruments, highly recommend.
Rating: 4.7

  • Application Area:Cell killing assay

"It's a great machine, easy to use, highly efficient, stable and data consistent, but E-plate is too expensive to afford."

Review date: 18 Jul 2018 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Sanofi



  • Ease of use
    4 out of 5
    After sales service
    1 out of 5
    Value for money
    3 out of 5
I can live without this instrument, but life would be poorer.
Rating: 2.7

  • Application Area:Cellular assays

"xCelligence Technology is highly interesting. The flexibility of use is not that brilliant. Furthermore the throughput is low, as there is a strong effect with outside wells, shrinking a 96-well to 60-wel.l"

Review date: 18 Jul 2018 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: FISABIO



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
We are delighted with this device!
Rating: 5.0

  • Application Area:Quantification of bacterial biofilm formation in real time

"It has allowed us to observe in real time how are the dynamics of biofilm formation in real time in an easy and comfortable way, without almost manipulating the sample. We conclude that it is an accurate and reproducible method, after assessing specifically the experiments reproducibility. In summary, this device opens a wide range of possibilities for the study of the wonderful world of biofilms, as well as a valuable tool for the effective treatment selection of diseases caused by bacterial biofilms."

Review date: 07 Nov 2017 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Hacettepe University Center for Stem Cell Research and Development



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rapid results, easy to use
Rating: 4.7

  • Application Area:Real time monitoring of cell growth

"Nice instrument, easy to calibrate, easy to use, direct comparison of growth rates between adherent cells using different culture conditions, including testing of effects of drugs and chemicals. System requires special culture plates, and uses impedance as a measurement of cell growth."

Review date: 02 Feb 2016 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: CarMen Laboratory



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:analyse complex professes including morphological changes, adhesion force and cell number

"-Highly reproducible -High reduction of time consuming to produce numerous data ; One experiment = one publication -Allows to check quality of cell batch -Allows to precisely identify times for complementary analyses -Requires small samples of either cells and media - Other Applications to study differentiating processes, real-time lipid uptake, co-cultures, adhesion…. "

Review date: 10 Jul 2015 | xCELLigence RTCA SP


  • Status:

    Reviewer

  • Member since: 2015

  • Organization: JCU



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:Monitoring cell growth from growth factors and cell deaths

"I love the ease of use and ability to overlap experiments with the one unit. The quick reads are invaluable for out toxin work and the automated nature means it is great to set up on a Friday and see cell growth results Monday morning. Costs are getting better in Australia as new distribution agreements and cheaper PET e-plates have helped immensely."

Review date: 30 Jun 2015 | xCELLigence RTCA SP
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Eli Lilly



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:Myotubular differentiation/fusion

"Extremely useful method for objectively quantifying myotube differentiation and fusion. Previous methods were either semiquantitative or did not take the entirety of morphological changes into account. xCELLigence allows us to measure these changes in real time, with complete accuracy, and full objectivity."

Review date: 30 Jun 2015 | xCELLigence RTCA SP

Label-free, real-time cell analysis in a 96-well format.

The xCELLigence® RTCA SP instrument uses noninvasive electrical impedance monitoring to quantify cell proliferation, morphology change, and attachment quality in a label-free, real-time manner.  The SP (single plate) model differs from our other xCELLigence instruments in that it uses a 96-well electronic microtiter plate (E-Plate® 96).  The instrument is placed in a standard CO2 cell culture incubator and interfaces via a cable with analysis and control units that are housed outside the incubator.  User friendly software allows for real-time control and monitoring of the instrument, and includes real-time data display and analysis functions. 

By skipping the guesswork associated with end-point assays and eliminating the time- and labor-intensive steps of traditional cell-based assays, real-time cell analysis (RTCA) with the xCELLigence RTCA SP vastly improves efficiency and overall productivity.  By eliminating the need for labels/dyes, the physiological relevance of acquired data is maximized.  The xCELLigence RTCA SP is currently being used worldwide in industrial and academic labs in applications that include, but are not limited to, the following:

  • Drug-Mediated Cytotoxicity/Apoptosis
  • Cell-Mediated Cytotoxicity and ADCC
  • GPCR-, NHR-, and RTK-Mediated Cell Signaling
  • Virus-Mediated Cytopathic Effects
  • Wound Healing
  • Endothelial Barrier Function
  • Stem Cell Differentiation
  • Immune Cell Activation
  • Phenotypic Screening
  • Cell Quality Control
  • Cell Adhesion and Spreading
  • Cell Response Profiling: Small Molecule & siRNA
  • Cell-Cell Interactions: Co-Culture

Product Overview

xCELLigence RTCA SP by ACEA Biosciences – A part of Agilent product image

xCELLigence RTCA SP

Manufacturer ACEA Biosciences – A part of Agilent  |  Available Worldwide

4.2 / 5.0 | 12 reviews