xCELLigence RTCA DP by ACEA Biosciences – A part of Agilent

Manufacturer ACEA Biosciences – A part of Agilent  |  Available Worldwide
4.3
/
5.0
  |  21 reviews
Cell invasion and migration measured in real-time. The xCELLigence® RTCA DP instrument uses noninvasive electrical impedance monitoring to quantify cell proliferation, morphology change, and attachment quality in a label-free, real-time manner.  What distinguishes the DP (dual purpose) model from our other xCELLigence instruments is its ability to additionally make kinetic measurements of cell invasion and migration (CIM) using an electronically integrated Boyden chamber (CIM-Plate® 16).  The... Read more


xCELLigence RTCA DP by ACEA Biosciences – A part of Agilent product image
xCELLigence RTCA DP
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Average Rating: 4.3
21 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2013

  • Organization: CEGMR



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
An instrument with great potential.
Rating: 4.7

  • Application Area: Cells migration/invasion assay, cytotoxicity, cells adherence and proliferation assays etc.

"The machine is easy to use with a very well written manual and comes with a user friendly software. The results I obtained from my experiments on the RTCA DP system were interesting and as expected. I would recommend it for cellular biology assays."

Review date: 18 Feb 2019 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Tulane University School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This machine is Awesome. Use it daily.
Rating: 5.0

  • Application Area:Real time label free cellular analyses

"xCELLigence is incredible. So easy to use. Very user friendly. It also can be used for almost ANY cell type: unlike other systems it does not require intact monolayers. Also, something like 70% of the well is electrode, making it incredibly sensitive to even small fluctuations."

Review date: 30 Aug 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Smith and Nephew Biotherapeutics



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Easy to use and produces good data!
Rating: 4.3

  • Application Area:Analyze the effect of drug substance on cell proliferation

"The xCELLigence system has been a huge asset to our lab. It is great to be able to monitor our cells and gather data in real time instead of end point assays. It gives us a better understanding of how the cells are behaving and greater insight into our research."

Review date: 31 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: University of Saskatchewan



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Excellent investment with reliable results.
Rating: 4.0

  • Application Area:Cell Poliferation

"This is one of the best and highly sensitive instrument for cell proliferation assays. We use this instrument a lot. The ease of setting up the assay and watch the resulting graphs without having to do anything for days together is simply awesome. Unlike end-point assays this continuous monitoring of cell growth/death gives a better understanding of the biological process. The highlight of this instrument is its small and does not take up the whole incubator space. The software used is also very user friendly and setting up the assay does not take more than couple of minutes. The only downside of this instrument is the cost of the plates used."

Review date: 30 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Medical University of Vienna, Ear Nose and Throat Department



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Never had problems, perfect results!
Rating: 5.0

  • Application Area:Analyse cell responses of primary human nasal epithelial cells

"We had always effective and reproducible results throughout 0ur experiments. We never had device problems. xCELLigence RTCA DP is very easy to use and easy to clean. After sales care was good and the device is for sure worth the money."

Review date: 26 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: University of Bern



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Great device, annoying software
Rating: 3.7

  • Application Area:Proliferation and migration assays with cultures cells

"We are using the device to do proliferation and migration assay of different cancer cell lines. The device is easy to handle. Although, I don't like the software of the Xcelligence. Espacially that you can't safe the data analysis part and also that you always have to click newly what you want to see on the screen, when you open a new file. Most of our results are reproducable. Sometimes I find it difficult to interpret the results. Therefore, I would find it cool when there would be some kind of forum where users could discuss their problems and exchange tipps and tricks. Otherwise the support from the OLS in Basel is very good, they always answer our questions fast and detailed."

Review date: 23 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Tulane University School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
One of the best additions to my lab in the last decade
Rating: 5.0

  • Application Area:cell adhesion, proliferation and barrier function

"This system is frankly brilliant. The interface is wonderfully user friendly, and the data incredibly reproducible. Great value for money, and easy to use. This system works really well also with cells that do not form monolayers, making it much more than just an ECIS system."

Review date: 19 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: University



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The real-time data akquise is great to determine the optimal timepoint.
Rating: 4.7

  • Application Area:Proliferation; Migration Invasion assays

"Verry helpful for non invasive meassurement of proliferation, invation and migration assays. High reproducible Results. The software is easy to use."

Review date: 19 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Dankook University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Great companion for (stem) cell study in real-time
Rating: 4.0

  • Application Area:Cell growth and behavior analysis upon chemicals and nanomaterial treatment

"Lable-free and real-time analysis of cell growth and behavior made simple with xCELLIgence RTCA DP. Good for short term response as well as long-term behaviour. Can accomodate various cell lines which showed consistent results. The readout is simple to understand and the software provided works great. Although the E-plate is expensive, you can get solid results, so value for money is not bad. I got after sales care for the first two years, which would be enough because the machine is not complicated and does not need much care except regular cleaning and common sense maintenance."

Review date: 19 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: University Hospital Würzburg, Department of Neurosurgery



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Once running by experienced user all is great. German customer care could be improved.
Rating: 3.7

  • Application Area:Testing chemotherapeutics on glioblastoma cells, measuring proliferation and migration

"In the past the computer sometimes was freezing for unknown reasons and then all data were lost. However, recently everything runs smoothly. Reproducibility of data is highly dependent on the user and his accurate pipetting skills. Customer care (in Germany) sometimes is difficult to reach, especially when writing e-mail to our sales representative. However, if reached, support is very competent and helpful."

Review date: 19 Jul 2018 | xCELLigence RTCA DP


  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Solvay



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    5 out of 5
Very interesting and promising tool from fundamental research to screening approaches
Rating: 4.0

  • Application Area:Toxicology

"Be creative and this equipement will feel your expectations"

Review date: 19 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: National cancer Institute of Naples



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
good results
Rating: 3.7

  • Application Area:Cell migration, matrigel invasion, trans-endothelial and mesothelial migration, proliferation

"It is a valid instrument for 2D models. importantly, the obtained data may be analyzed obiectively"

Review date: 18 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: INSERM U1160



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Very sensitive assay to measure cell mediated lysis of tumor cells
Rating: 4.3

  • Application Area:resistance to kinase inhibitors of cancer cells, immune cell mediated lysis assay

"We use the ACEA system to follow growth of tumors cells in response to kinase inhibitors, to check resistance to drugs and inhibitors. We use the ACEA system for cell mediated lysis assay: an assay we have set up several years ago and routinelly performed in the lab"

Review date: 18 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Institut pasteur



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Great, reproducible, fast. Software could be improved
Rating: 4.0

  • Application Area:titration of virus samples

"The results produced with this device are highly reproductible. You can analyse many different samples in one experiments. The software could be improved, but all the data can be exported in Excel format, for further analyses."

Review date: 18 Jul 2018 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Center for Cancer Immune Therapy



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The fact that you can monitor killing over multiple days makes it a must-have
Rating: 5.0

  • Application Area:Cellular immunotherapy

"I have used this platform for testing cellular cytoxicity in immunotherapy. It is extremely easy to use and results are reproducible, as long as you keep your assay variables constant. Otherwise the high sensitivity of the platform will play tricks on you. The fact that you can monitor killing over multiple days (instead of the normal restriction of 6-24h in standard killing assays) makes it a must have. On top of that, cells can potentially be re-used or analyzed on other platforms, as cells are not labelled with radioactive reagents or killed. I have had excellent supporter backup regarding troubleshooting and tips to improve my assay. All in all, this platform is highly recommendable for studying cellular toxicity."

Review date: 13 Oct 2017 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The University of Melbourne



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Overall, the xCELLigence DP is a breeze to use and enables you to generates loads of data quickly.
Rating: 4.0

  • Application Area:Investigate functional responses of cells to various stimuli

"The xCELLigence DP is easy to use, provides the flexibility of monitoring cells in real-time and allows the assessment of multiple time points and dual functions in a single experiment, without the need to repeat tedious, conventional endpoint assays numerous times. The small reagent volumes required supports the economical use of limited reagents. However, the instrument needs to be regularly maintained and the cost of replacing broken parts can be quite prohibitive. Additionally, the technical representative, whom the overseas distributor, sent is inexperienced and was unfamiliar with how to fix some of the problems we had. Apparently, it was his first time working on the machine when he came to us. "

Review date: 23 Jun 2016 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: IT Sligo



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great user friendly system for a more dynamic and sensitive assessment of cell growth in real time.
Rating: 4.3

  • Application Area:cell growth response to novel anti-cancer chemotherapeutics

"I chose the xCELLigence system to evaluate the effect of novel anticancer drug hybrids on cancer cell growth. Overall the system was very user friendly and graphs generated were intuitive, informative and reproducible. This platform offered several advantages specific to my research needs. Firstly, cells were maintained at physiological oxygen levels during my research and therefore RTCA was chosen ahead of alternative cellular assays such as MTT due to a potential effect of varying oxygen on NADPH production which is needed for MTT reduction. Furthermore, RTCA detected not only drug-induced changes in cell number but also the kinetics of drug action. RTCA was invaluable in defining optimal seeding densities and drug concentration range and enabled us to detect instances of cell recovery which may otherwise have been overlooked. The IC50 function was also useful in surmising overall activity of novel anticancer drugs. "

Review date: 23 Jun 2016 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Oregon State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Awesome machine easy to use with great results ........ it will help me to graduate.
Rating: 5.0

  • Application Area:Nanoparticle Drug Delivery

"I thoroughly love this machine we have used it twice in two of my first author publications. It is a simple setup with reproducible and reliable results. I have found if something went wrong it was more than likely the user not the machine. The data is clean and easily interpreted. In all 10 out of 10 would recommend."

Review date: 31 May 2016 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Hudson Institute of Medical Research



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
This simple to use instrument has revolutionized my experimental workflow.
Rating: 4.7

  • Application Area:3D Co-Culture Invasion

"This simple to use instrument has revolutionized my experimental workflow. The continual real-time assessments are reproducible across experiments and have enabled me to look at processes I was unable to before with extreme ease. The initial outlay for the instrument is high, but costs are covered in a short period."

Review date: 21 Jul 2015 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: IFIB (Eberhard Karls Universität Tübingen)



  • Ease of use
    4 out of 5
    After sales service
    2 out of 5
    Value for money
    4 out of 5
It could help us to solve various questions for different types of cells.
Rating: 3.3

  • Application Area:Application: Cell growth and migrations

"Our lab is using the xCELLigence DP device pretty often. It could help us to solve various questions for different types of cells. Some of this questions could not be answered by common end-Point assays, why we made some new findings. Once we had the problem, that the USB port of our device started to get rusty although we were using the device normally in our incubator. From our point of view, something like this should not happen, because the xCELLigence device is made for using it under normal conditions, which includes the CO2 incubator. Therefore we called the Service. They made us an offer for about 200 € to exchange the USB-port. We rejected this offer, because we felt that this USB-port is an error made by the Company and not our mistake (with normal use). Expect from the problem with the USB-port, we would recommend to everybody interested in cell behaviour to buy this xCELLigence device. And we are advertising the use of it, to other persons. "

Review date: 17 Jul 2015 | xCELLigence RTCA DP
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Sanford Research



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:We analyze the effect of potential cancer therapies on cancer cells

"Pretty easy to use, love that you can get data while you're out of the lab. Will be publishing data soon."

Review date: 30 Jun 2015 | xCELLigence RTCA DP

Cell invasion and migration measured in real-time.

The xCELLigence® RTCA DP instrument uses noninvasive electrical impedance monitoring to quantify cell proliferation, morphology change, and attachment quality in a label-free, real-time manner.  What distinguishes the DP (dual purpose) model from our other xCELLigence instruments is its ability to additionally make kinetic measurements of cell invasion and migration (CIM) using an electronically integrated Boyden chamber (CIM-Plate® 16).  The three cradles of the DP instrument enable three separate electronic 16-well plates to be controlled and monitored in parallel or independently of one another, allowing maximal productivity for multiple users.  The instrument is placed in a standard CO2 cell culture incubator and is powered and controlled via a cable connected to the control unit (a laptop computer) housed outside the incubator.  User friendly real-time cell analysis (RTCA) software allows for real-time interfacing with all three cradles, and includes real-time data display and analysis functions. 

By skipping the guesswork associated with end-point assays and eliminating the time- and labor-intensive steps of traditional methods, real-time cell analysis with the xCELLigence RTCA DP vastly improves efficiency and overall productivity.  It is currently being used worldwide in industrial and academic labs in applications that include, but are not limited to, the following:

  • Cell Invasion and Migration
    • Cancer Metastasis
    • Inflammation
    • Wound Healing
    • Embryo Implantation
  • Drug-Mediated Cytotoxicity/Apoptosis
  • Cell-Mediated Cytotoxicity and ADCC
  • GPCR-, NHR-, and RTK-Mediated Cell Signaling
  • Virus-Mediated Cytopathic Effects
  • Endothelial Barrier Function
  • Stem Cell Differentiation
  • Immune Cell Activation
  • Phenotypic Screening
  • Cell Quality Control
  • Cell Adhesion and Spreading
  • Cell Response Profiling: Small Molecule & siRNA
  • Cell-Cell Interactions: Co-Culture

Product Overview

xCELLigence RTCA DP by ACEA Biosciences – A part of Agilent product image

xCELLigence RTCA DP

Manufacturer ACEA Biosciences – A part of Agilent  |  Available Worldwide

4.3 / 5.0 | 21 reviews